A system and method for automated single cell capture and processing is described, where the system includes a deck supporting and positioning a set of sample processing elements; a gantry for actuating tools for interactions with the set of sample processing elements supported by the deck; and a base supporting various processing subsystems and a control subsystems in communication with the processing subsystems. The system can automatically execute workflows associated with single cell processing, including mRNA capture, cDNA synthesis, protein-associated assays, and library preparation, for next generation sequencing.

Reaction vessels, cartridges, devices and methods for facilitating high-level multiplexing are described herein. Such reaction vessels can include a planar frame defining a fluidic path between a first planar substrate and a second planar substrate, a fluidic interface is located at one end of the planar frame with a pair of fluidic ports, a well chamber and a pre-amplification chamber. Devices for spotting reagents in wells of high-level multiplexing reaction vessels and improved reagent solutions are also described herein.

The present invention has: a step for heating a resin or a metal in a state in which the resin or metal is sandwiched by a mold provided with a projection that is constituted from an elastic body having a heat resistance temperature higher than the temperature at which the resin or the metal softens, and a support body which is larder than the mold and which is softened by heat at a higher temperature than the resin or the metal, while a force is applied to the mold in the direction toward the resin or the metal.

An apparatus for gene amplification includes a gene amplification chip including a well configured to accept a sample that is loaded into the well; the gene amplification chip being configured to: thermally dissolve the sample in the well so that a microbe present in the sample is thermally dissolved in the well to release genes in the microbe; and amplify the released genes in the well. The apparatus for gene amplification also includes a temperature controller configured to control a thermal dissolution temperature and a gene amplification temperature of the well.

A system for performing biological reactions is provided. The system includes a chip including a substrate and a plurality of reaction sites. The plurality of reaction sites are each configured to include a liquid sample of at most one nanoliter. Further, the system includes a control system configured to initiate biological reactions within the liquid samples. The system further includes a detection system configured to detect biological reactions on the chip. According to various embodiments, the chip includes at least 20000 reaction sites. In other embodiments, the chip includes at least 30000 reaction sites.

A microfluidic device is disclosed which comprises a main flow channel and a partition chamber connected to a portion of same by a chamber inlet and chamber outlet. The device utilizes select cross sections to advantage capillary effects during filling and partitioning steps to isolate biological or other samples in the partition chamber for analysis and can be employed in a digital array.

The present invention is in the field of microfluidic devices produced with silicon technology wherein at least one 3D microenvironment is present, a method of producing said device using silicon based technology, and a use of said device in various applications, typically a biological cell experiment, such as a cell or organ on a chip experiment, and use o the device as a microreactor.

In an example method, a series of time-based clustering images is generated for a plurality of library fragments from a genome sample. Each time-based clustering image in the series is sequentially generated. To generate each time-based clustering image in the series: i) a respective sample is introduced to a flow cell, the respective sample including contiguity preserved library fragments of the plurality of library fragments, wherein the contiguity preserved library fragments are attached to a solid support or are attached to each other; ii) the contiguity preserved library fragments are released from the solid support or from each other; iii) the contiguity preserved library fragments are amplified to generate a plurality of respective template strands; iv) the respective template strands are stained; and v) the respective template strands are imaged.

A receiving unit for receiving a fluid has a receiving element with a receiving face and at least one micro-cavity that is arranged and formed in the receiving element on the receiving face in order to receive the fluid. The receiving face further has a hydrophilic surface characteristic in at least one subregion adjoining the at least one micro-cavity.

A detection chip, a method of using a detection chip and a reaction system are provided. The detection chip includes a first substrate, a micro-chamber definition layer and a heating electrode. The micro-chamber definition layer is located on the first substrate and defines a plurality of micro-reaction chambers. The heating electrode is located on the first substrate and closer to the first substrate than the micro-chamber definition layer, and configured to release heat after being energized. The heating electrode includes a plurality of sub-electrodes, orthographic projections of the plurality of micro-reaction chambers on the first substrate overlap with orthographic projections of at least two of the plurality of sub-electrodes on the first substrate, and the at least two of the plurality of sub-electrodes have different heating values per unit time after being energized.