Devices and methods for controlling collection of liquid sample are described. In an example, a microfluidic device can include an analytical device and an actuator. The actuator can be connected to the analytical device. The actuator can be operable to absorb fluid. The actuator can guide the absorbed fluid to an input layer of the analytical device. The actuator can deform in response to an occurrence of an absorption condition. A degree of deformation of the actuator indicates a volume of fluid collected by the analytical device.

The present disclosure relates to a method for optimizing a measurement rate of a field device in a measurement system. The measurement system includes at least one second field device in which a measurement variable of the field device is correlated with the measurement variable of the second field device. The method determines a respective specific correlation pattern between the first measurement variable and the second measurement variable based on a learning phase. This makes it possible to check the measured values from the second field device for the correlation pattern during normal measurement operation and to change the measurement rate of the field device during the corresponding time window. This makes it possible to increase the service life and/or availability in the process installation.

Systems and methods for measuring dynamic hydraulic conductivity and permeability associated with a cell layer are disclosed. Some systems include a microfluidic device, one or more working-fluid reservoirs, and one or more fluid-resistance element. The microfluidic device includes a first microchannel, a second microchannel, and a barrier therebetween. The barrier includes a cell layer adhered thereto. The working fluids are delivered to the microfluidic device. The fluid-resistance elements are coupled to one or more of the fluid paths and provide fluidic resistance to cause a pressure drop across the fluid-resistance elements. Mass transfer occurs between the first microchannel and the second microchannel, which is indicative of the hydraulic conductivity and/or dynamic permeability associated with the cells.

Microfluidic structures and methods for manipulating fluids, fluid components, and reactions are provided. In one aspect, such structures and methods can allow production of droplets of a precise volume, which can be stored/maintained at precise regions of the device. In another aspect, microfluidic structures and methods described herein are designed for containing and positioning components in an arrangement such that the components can be manipulated and then tracked even after manipulation. For example, cells may be constrained in an arrangement in microfluidic structures described herein to facilitate tracking during their growth and/or after they multiply.

The present disclosure provides apparatuses, systems, and methods for performing particle analysis through flow cytometry at comparatively high event rates and for gathering high resolution images of particles.

A process and system for efficiently producing reference data that can be fed into a predictive model for predicting quality attribute concentrations in cell culture processes. A perfusion bioreactor is operated at pseudo-steady-state conditions and one or more attribute influencing parameters are manipulated and changed over time. As the one or more attribute influencing parameters are manipulated, one or more quality attributes are monitored and measured. In one embodiment, multiple quality attributes are monitored and measured in parallel. The quality attribute information is recorded in conjunction with the changes in the attribute influencing parameters. This information is then fed to the predictive model for propagating cell cultures in commercial processes and maintaining the cell cultures within desired preset limits.

A system for testing for an analyte includes a test strip. The test strip includes a first flow path. The test strip further includes a heating element in communication with a heating area of the first flow path, for heating a sample in the first flow path. The test strip further includes an e-gate, the e-gate in the first flow path, the e-gate separating the heating area from a detection area of the first flow path.

A point of use analyzer includes pump, valve, port, and storage channel. The storage channel may hold multiple assay packets composed of reagent aliquots separated by bounding slugs. The storage channel may define an elongated lumen having two ends with each of the ends coupled to the valve. A sampling device for use with the analyzer engages the port and may include a recurrent coaxial tube having a separation medium. A method of using the analyzer with the sampling device includes steps of pumping a fluid to displace a sample into the separation medium and out through the opposed connection.

The present disclosure provides apparatuses, systems, and methods for performing particle analysis through flow cytometry at comparatively high event rates and for gathering high resolution images of particles.

An immunoassay device for use in quantitatively measuring an amount of an analyte in a fluid sample, employs reagents that include particle pairs comprising a) one of an antigen and antibody coupled with a label, and b) a magnetic particle coupled with the other of the antigen and antibody. A transport which moves a set of reaction wells along a path and a dispenser dispenses respective ones of the reagents into the reaction wells. Prior to magnetic separation and optical analysis, a controller that coordinates movement of the transport with operation of the pipette modules operates the transport to reciprocate the set of reaction wells along the path for mixing the fluid sample with the reagents.