Disclosed are a dilution method and a dilution apparatus. The dilution method includes: adding a sample into a first reactor at a first station of a supply unit; transferring the first reactor to a fifth station of a transit apparatus, and receiving a second reactor at the first station of the supply unit; adding a diluent into the first reactor at the fifth station to obtain a diluted sample; uniformly mixing the diluted sample in the first reactor; transferring the first reactor from the transit apparatus to a dilution transport apparatus; transferring part of the diluted sample in the first reactor to the second reactor; transferring the second reactor to the fifth station of the transit apparatus, and continuing to add the diluent into the second reactor; and uniformly mixing substances in the second reactor. The dilution apparatus includes the supply unit and the transit apparatus.

A fluid disposing system includes a centrifugal separator that centrifugally separates a liquid that is supplied, a reagent injecting apparatus coupled to the centrifugal separator and that injects a reagent into the centrifugal separator, a reagent supply module that supplies the reagent to the reagent injecting apparatus and a pipetting module provided on an upper side of the centrifugal separator and that feeds the fluid to the centrifugal separator.

Methods of making and using a magnetic ECM are disclosed. The ECM comprises positively and negatively charged nanoparticles, wherein one of said nanoparticles contains a magnetically responsive element. When the magnetic ECM is seeded with cells, the cells will be magnetized and can be levitated for 3-D cell culture.

Disclosed herein is a novel method to fabricate magnetic silica nanomembranes using thin polymer cores based on silica deposition and self-wrinkling induced by thermal shrinkage. These micro- and nano-scale structures have vastly enlarged the specific area of silica, thus the magnetic silica nanomembranes can be used for solid phase extraction of nucleic acids. The magnetic silica nanomembranes are suitable for nucleic acid purification and isolation and demonstrated better performance than commercial particles in terms of nucleic acid recovery yield and integrity. In addition, the magnetic silica nanomembranes may have high nucleic acid capacity due to significantly enlarged specific surface area of silica. Methods of use and devices comprising the magnetic silica nanomembranes are also provided herein.

A fluidic device includes: a first flow path in which two or more solutions are mixed; and a second circulation flow path in which a solution mixed in the first flow path is circulated and which has a capture part configured to capture a sample substance included in the solution and/or a detection part configured to detect a sample substance included in the solution.

The invention relates to a system, comprising: a) a sample processing unit, comprising an input port and an output port coupled to a rotating container having at least one sample chamber, the sample processing unit configured provide a first processing step to a sample or to rotate the container so as to apply a centrifugal force to a sample deposited in the chamber and separate at least a first component and a second component of the deposited sample; and b) a sample separation unit coupled to the output port of the sample processing unit, the cell separation unit comprising separation column holder (42), a pump (64) and a plurality of valves (1-11) configured to at least partially control fluid flow through a fluid circuitry and a separation column (40) positioned in the holder, the separation column configured to separate labeled and unlabeled components of sample flowed through the column.

Devices, systems, and methods for applying a dielectrophoretic force on a particle include: a cell defining at least one channel for confining the particle; and a first electrode and a second electrode electrically isolated from the first electrode, at least one of the first and second electrodes being formed from a two-dimensional (2D) material providing an atomically sharp edge. The first and second electrodes are arranged sufficiently close to one another and sufficiently close to the channel such that application of a sufficient voltage across the first and second electrodes generates an electric field in at least part of the channel, the electric field having an electric field gradient sufficient to apply the dielectrophoretic force on the particle in the channel.

An apparatus and method for isolating bacterial particles in a sample using a container with material in temporary fluid blocking position to lower orifice in the container, a separation medium having an electrical conductivity lower than and physical density greater than that of the sample above the material that supports a sample concentrate after passing through the separation medium when exposed to centrifugal force, a heating element for liquefying the material to permit flow into a chamber past an electrode array that attracts and holds subject particles. The system allows rapid detection and isolation of particles from samples from animal, human, environmental sites, a bio-industrial reactor or a food or beverage production facility requiring relatively small volumes, short incubation times resulting in structurally intact particles for further analysis. Testing may be completed in a single unit that requires decreased technician manipulation, fewer steps and a decrease in cross-contamination.

Devices and methods for performing dielectrophoresis are described. The devices contain sample channel which is separated by physical barriers from electrode channels which receive electrodes. The devices and methods may be used for the separation and analysis of particles in solution, including the separation and isolation of cells of a specific type. As the electrodes do not make contact with the sample, electrode fouling is avoided and sample integrity is better maintained.

A dielectrophoretic detection device including a chip, with a flow channel having at least one inlet and one outlet, and at least a detection area configured to detect analytes trapped on functionalised beads flowing within the flow channel, first and second electrode assemblies shaped as rows of parallel pillars extending a the height of the flow channel, and configured to generate under electric tension an electric field to form an electrical barrier, and preventing the beads to cross the barrier and drawing the beads to the detection area by dielectrophoretic forces where they are clustered and concentrated. The device may be provided with multiple rows of parallel pillars of electrode assemblies extending over the height of the flow channel, forming multiple concentration lines. The flow channel may be provided with further rows of parallel pillars of electrode assemblies crossing the flow channel in a transverse direction, forming further incubation lines.