A method for producing a mutant filamentous fungus. The method comprises modifications of XYR1 and ACE3 expression in the parent filamentous fungus. The modification of XYR1 is substitution, deletion, insertion, or addition of at least one amino acid residue in a region corresponding to positions 810 to 833 of SEQ ID NO: 1 in a polypeptide that consists of the amino acid sequence of SEQ ID NO: 1 or an amino acid sequence having at least 90, identity thereto and functions as a transcriptional activator of cellulase and hemicellulase, and the modification of ACE3 expression is enhanced expression of a partial polypeptide of ACE3.

A method for producing a mutant filamentous fungus. The method comprises modifications of XYR1 and ACE3 expression in the parent filamentous fungus. The modification of XYR1 is substitution, deletion, insertion, or addition of at least one amino acid residue in a region corresponding to positions 810 to 833 of SEQ ID NO: 1 in a polypeptide that consists of the amino acid sequence of SEQ ID NO: 1 or an amino acid sequence having at least 90, identity thereto and functions as a transcriptional activator of cellulase and hemicellulase, and the modification of ACE3 expression is enhanced expression of a partial polypeptide of ACE3.

The present invention concerns a nucleic acid molecule capable of expressing, in at least one plant tissue, a chimeric protein comprising a polygalacturonase (PG) of fungal, bacterial or insect origin and a plant polygalacturonase inhibitor protein (PGIP) plant capable of inhibiting said PG. The present invention also relates to transgenic plants that express said chimeric protein.

The present invention concerns a nucleic acid molecule capable of expressing, in at least one plant tissue, a chimeric protein comprising a polygalacturonase (PG) of fungal, bacterial or insect origin and a plant polygalacturonase inhibitor protein (PGIP) plant capable of inhibiting said PG. The present invention also relates to transgenic plants that express said chimeric protein.

There is described a continuous process for producing and isolating mycoprotein. The process may comprise the steps of: providing a fermentation media suitable for producing mycoprotein; introducing the fermentation media to a first fermentation vessel; fermenting the fermentation media to obtain a mixture comprising mycoprotein and partially spent fermentation media; isolating at least part of the partially spent fermentation media from the mixture comprising mycoprotein and partially spent fermentation media; and reintroducing at least a portion of the isolated partially spent fermentation media into the first fermentation vessel. Also described is mycoprotein obtained from the process.

There is described a continuous process for producing and isolating mycoprotein. The process may comprise the steps of: providing a fermentation media suitable for producing mycoprotein; introducing the fermentation media to a first fermentation vessel; fermenting the fermentation media to obtain a mixture comprising mycoprotein and partially spent fermentation media; isolating at least part of the partially spent fermentation media from the mixture comprising mycoprotein and partially spent fermentation media; and reintroducing at least a portion of the isolated partially spent fermentation media into the first fermentation vessel. Also described is mycoprotein obtained from the process.

The invention relates to a strain of fungus having a reduced viscosity, wherein the ID 78713 (GEL3) gene has been invalidated. The invention also relates to the different uses of this strain, as well as to the method of genetic modification.

The invention is directed to methods of diagnosing a Pseudoperonospora cubensis infection in a cucurbit plant, comprising detecting at least one nucleotide sequence selected from the group of nucleotide sequences of SEQ ID NOs: 1-52, or any combination thereof, in a sample from a cucurbit plant or in an environmental sample, thereby diagnosing a P. cubensis infection. Also provided is a method of selecting a cucurbit plant comprising at least one resistance gene, the method comprising introducing into the cucurbit plant a nucleotide sequence of any one of SEQ ID NOs: 1-52, wherein the expression of the nucleotide sequence produces a hypersensitive response in a cucurbit plant comprising a resistance gene, thereby identifying the cucurbit plant as comprising a resistance gene; and selecting the plant having the hypersensitive response and identified as comprising said resistance gene.

The invention is directed to methods of diagnosing a Pseudoperonospora cubensis infection in a cucurbit plant, comprising detecting at least one nucleotide sequence selected from the group of nucleotide sequences of SEQ ID NOs: 1-52, or any combination thereof, in a sample from a cucurbit plant or in an environmental sample, thereby diagnosing a P. cubensis infection. Also provided is a method of selecting a cucurbit plant comprising at least one resistance gene, the method comprising introducing into the cucurbit plant a nucleotide sequence of any one of SEQ ID NOs: 1-52, wherein the expression of the nucleotide sequence produces a hypersensitive response in a cucurbit plant comprising a resistance gene, thereby identifying the cucurbit plant as comprising a resistance gene; and selecting the plant having the hypersensitive response and identified as comprising said resistance gene.

The present invention belongs to the field of biotechnology, in particular to a multivalent fusion protein AB-NAC-189, method for producing the same, and uses thereof. The protein AB-NAC-189 is a fusion of a polypeptide segment AB, nascent polypeptide-associated complex (NAC), and a protein 189 corresponding to amino acids 1-189 from the N-terminal of protein HarpinEa. The fusion has the properties of a multivalent plant immune protein, thus it can effectively stimulate the hypersensitive response of tobacco leaves and has good thermal stability. While stimulating the immune response of plants, it can also improve the disease resistance of plants and promote plant growth. The AB-NAC-189 multivalent vaccine shows higher activity per unit concentration, and greater ability to promote growth of wheat and tobacco; meanwhile it can significantly promote chlorophyll synthesis in Goji berry, thereby improving the yield and quality of Goji berries.