A mixed mode affinity chromatography carrier includes a substrate, a hydrophilic polymer, an antibody-binding cyclic peptide, and a cation exchange group.

The present invention relates to novel engineered artificial immunoglobulin (Ig) binding polypeptides comprising three domains and two linkers. The invention further relates to affinity matrices comprising these artificial Ig binding molecules of the invention. The novel Ig binding proteins are particularly useful for the affinity purification of proteins requiring elution at a higher pH (in particular higher than pH 4.2). The invention also relates to a use of the novel Ig binding proteins or affinity matrices for affinity purification of immunoglobulins and to methods of affinity purification using the novel Ig binding proteins of the invention.

The present invention relates to novel engineered artificial immunoglobulin (Ig) binding polypeptides comprising three domains and two linkers. The invention further relates to affinity matrices comprising these artificial Ig binding molecules of the invention. The novel Ig binding proteins are particularly useful for the affinity purification of proteins requiring elution at a higher pH (in particular higher than pH 4.2). The invention also relates to a use of the novel Ig binding proteins or affinity matrices for affinity purification of immunoglobulins and to methods of affinity purification using the novel Ig binding proteins of the invention.

Amyloid fibrils comprising pathogen binding proteins and methods of their use are provided.

Amyloid fibrils comprising pathogen binding proteins and methods of their use are provided.

The objective of the present invention is to provide an affinity separation matrix having excellent adsorption performance and binding capacity to a peptide containing a Fab region of IgG, and a method for producing a Fab region-containing peptide using the affinity separation matrix. The affinity separation matrix according to the present invention is characterized in that a Fab region-binding peptide is immobilized as a ligand on a water-insoluble carrier in a density of 1.0 mg/mL-gel or more.

One embodiment of the present invention is a protein having affinity for an immunoglobulin, which is a protein having two or more domains derived from any of the amino acid sequences of E, D, and A domains of protein A, and in the amino acid sequence of at least one of the domains, one or more lysines are included, and the C-terminal lysine is deleted or substituted, or a protein having affinity for an immunoglobulin, which is a protein having two or more domains derived from any of B, C, and Z domains of protein A, and in the amino acid sequence of at least one of the domains, one or more lysines are included, and lysine at position 4 and the C-terminal lysine are deleted or substituted.

One embodiment of the present invention is a protein having affinity for an immunoglobulin, which is a protein having two or more domains derived from any of the amino acid sequences of E, D, and A domains of protein A, and in the amino acid sequence of at least one of the domains, one or more lysines are included, and the C-terminal lysine is deleted or substituted, or a protein having affinity for an immunoglobulin, which is a protein having two or more domains derived from any of B, C, and Z domains of protein A, and in the amino acid sequence of at least one of the domains, one or more lysines are included, and lysine at position 4 and the C-terminal lysine are deleted or substituted.

Constructs having membrane proteins stabilized by functionalized beta-sheet peptides are provided. The constructs can be associated with or covalently linked to supports. The support can be a membrane. The membrane can be used to selectively move desired particles from one side of the membrane to the other while impeding passage of undesired particles through the membrane. Methods of making and using such constructs and membranes are provided.

Proteinaceous cellulose microparticles are provided. These microparticles comprise cellulose nanocrystals and one or more peptide, one or more protein, or a mixture thereof, wherein the nanocrystals and the peptide(s) and/or protein(s) are aggregated together to form the microparticles. In embodiments, the microparticles comprise silk fibroin and advantageously are hydrophobic and lipophilic. There are also provided cosmetic preparations comprising these microparticles. In advantageous embodiments, these cosmetic preparations comprise a water-in-oil emulsion or a lipophilic medium. Finally, there are also provided methods of producing the microparticles.