In one or more embodiments, the present invention relates to a method for producing a polyhydroxybutyrate-based resin. The method includes (a) disrupting or solubilizing microbial cells containing a polyhydroxybutyrate-based resin, and (b) separating the polyhydroxybutyrate-based resin from a composition obtained by the process (a). The process (a) and the process (b) use water with a calcium ion concentration (14.5 mg/L or less. The water used in the process (a) and the process (b) is preferably obtained by subjecting wastewater that is discharged from the production process of the polyhydroxybutyrate-based resin to microbial anaerobic and aerobic treatments, subsequently pre-filtration by a membrane bioreactor process, and further filtration with a calcium ion removal membrane This method provides the polyhydroxybutyrate-based resin with good color tone and high thermal stability.

A process for producing a polyester composition from recycled polyesters.

The method isolates polyhydroxyalkanoate (PHA) from a PHA-rich bacterial biomass, to the PHA isolated by the method and to a PHA having specific properties. The method treats an aqueous suspension of the PHA-rich bacterial biomass with a minimal amount of sodium hypochlorite, a methanol wash and extraction of PHA from the biomass with dimethyl carbonate (DMC).

The method isolates polyhydroxyalkanoate (PHA) from a PHA-rich bacterial biomass, to the PHA isolated by the method and to a PHA having specific properties. The method treats an aqueous suspension of the PHA-rich bacterial biomass with a minimal amount of sodium hypochlorite, a methanol wash and extraction of PHA from the biomass with dimethyl carbonate (DMC).

A method for producing a polyhydroxyalkanoate, the method including: (a) preparing an aqueous polyhydroxyalkanoate suspension having a pH of not more than 5; (b) mixing the aqueous suspension obtained in (a) with a water-insoluble organic solvent having a specific gravity of more than 1.0 g/mL; (c) separating a mixed solution obtained in (b) into a water-insoluble organic solvent phase and an aqueous phase by centrifugal separation, and then removing the aqueous phase; and (d) heating the water-insoluble organic solvent phase obtained in (c), and then cooling the water-insoluble organic solvent phase to obtain a gelatinous polyhydroxyalkanoate.

A method for producing a polyhydroxyalkanoate, the method including: (a) preparing an aqueous polyhydroxyalkanoate suspension having a pH of not more than 5; (b) mixing the aqueous suspension obtained in (a) with a water-insoluble organic solvent having a specific gravity of more than 1.0 g/mL; (c) separating a mixed solution obtained in (b) into a water-insoluble organic solvent phase and an aqueous phase by centrifugal separation, and then removing the aqueous phase; and (d) heating the water-insoluble organic solvent phase obtained in (c), and then cooling the water-insoluble organic solvent phase to obtain a gelatinous polyhydroxyalkanoate.

The present invention relates to methods for the preparation of high molecular weight polyhydroxybutyrate (PHB) by culturing Bacillus megaterium strains in a mixture of agri-food wastes, PHB obtained or obtainable by the methods as well as to its use in the preparation of articles such as, for example, soles and/or heels for shoes.

The present invention relates to methods for the preparation of high molecular weight polyhydroxybutyrate (PHB) by culturing Bacillus megaterium strains in a mixture of agri-food wastes, PHB obtained or obtainable by the methods as well as to its use in the preparation of articles such as, for example, soles and/or heels for shoes.

The present specification generally relates to a method for the oxidative conditioning of a microbial cell wall, and its eventual lysis. In particular, the specification pertains to an oxidative lysis solution and methodology, wherein the selective lysis solution is capable of conditioning and lysing the cell wall of polyhydroxyalkanoate-producing microbes, while having a limited effect on the degradation of the polyhydroxyalkanoate (PHA) biopolymer solids contained therein, thereby allowing for the further high yield recovery, and purification of high molecular weight PHA biopolymer solids.

The present invention relates to a method for producing biodegradable microspheres having improved safety and storage stability, and a method for producing the same. The present invention provides a method for preparing said biodegradable microspheres while minimizing the morphological changes of microspheres and significantly reducing residual solvents.