The invention refers to a method and a device for the phenotypic detection of carbapenemases and carbapenemase producers by adding a substrate of general formula A-(L)-M.sub.1-(X)—Z, where M.sub.1 is a carbapenem backbone, A or Z is a quencher, the other one of the two, Z or A, is a fluorophore, L is an optional linker, X is an optional leaving group for linking Z to the carbapenem backbone, and Z is an optional leaving group, to a sample suspected of containing such carbapenemase producers and/or carbapenenmases. The invention further refers to a method for the phenotypic detection of resistant bacteria, in particular 3MRGN or 4MRGN, by releasing the enzymes of a bacterial culture into a lysate during lysis and then subjecting the lysate, as the sample to be analyzed, to an aforementioned method in order to phenotypically detect the presence of resistance-conferring carbapenemases.

Novel rhodamine dye compounds, labelled conjugates comprising the dyes are described, together with methods for their use. The dyes and labelled conjugates are useful as molecular probes in a variety of applications, such as in assays involving staining of cells, protein binding, and analysis of nucleic acids, such as hybridization assays and nucleic acid sequencing.

Novel rhodamine dye compounds, labelled conjugates comprising the dyes are described, together with methods for their use. The dyes and labelled conjugates are useful as molecular probes in a variety of applications, such as in assays involving staining of cells, protein binding, and analysis of nucleic acids, such as hybridization assays and nucleic acid sequencing.

Zwitterionic luminescent polymeric nanoparticles, which include at least one luminescent dye and at least one random copolymer, and the method of preparing the zwitterionic luminescent polymeric nanoparticles. Also, the use of these zwitterionic luminescent polymeric nanoparticles in the medical field and in the biological research field for in vitro or in vivo detection or tracking of a target biological molecule.

Zwitterionic luminescent polymeric nanoparticles, which include at least one luminescent dye and at least one random copolymer, and the method of preparing the zwitterionic luminescent polymeric nanoparticles. Also, the use of these zwitterionic luminescent polymeric nanoparticles in the medical field and in the biological research field for in vitro or in vivo detection or tracking of a target biological molecule.

A composition includes a functionalized fluorescent dye. The functionalized fluorescent dye includes an isothiocyanate-containing dye that is functionalized with a functional group. The functional group includes a primary amine. The functionalized fluorescent dye can be mixed with a fluid to form a tracer fluid for tracing fluid flow in a subterranean formation.

A composition includes a functionalized fluorescent dye. The functionalized fluorescent dye includes an isothiocyanate-containing dye that is functionalized with a functional group. The functional group includes a primary amine. The functionalized fluorescent dye can be mixed with a fluid to form a tracer fluid for tracing fluid flow in a subterranean formation.

A novel non-fluorescent rhodamine dye forms a twisted intramolecular charge transfer state. A substituent that causes steric hindrance is introduced at an ortho position of a dimethylamino group on the xanthene ring of tetramethylrhodamine, which is a general rhodamine that exhibits strong fluorescence, and a certain amount of twist is imparted in a ground state. As a result, the formation of the twisted intramolecular charge transfer state is promoted in the excited state and non-fluorescence is exhibited.

A novel non-fluorescent rhodamine dye forms a twisted intramolecular charge transfer state. A substituent that causes steric hindrance is introduced at an ortho position of a dimethylamino group on the xanthene ring of tetramethylrhodamine, which is a general rhodamine that exhibits strong fluorescence, and a certain amount of twist is imparted in a ground state. As a result, the formation of the twisted intramolecular charge transfer state is promoted in the excited state and non-fluorescence is exhibited.

The disclosure belongs to the technical field of material science, and particularly relates to a method for preparing a high sensitivity temperature sensitive reversible color-changing microcapsule. According to the method, a series of novel fluorane dyes are designed and synthesized, the color-changing performance can be achieved without a color developing agent, the fluorane dyes are compounded with a phase change material according to a certain proportion, a series of two-component reversible temperature sensitive color-changing dyes are prepared, and then a two-component reversible thermochromic microcapsule with good color performance and color-changing response performance is prepared by adopting a solvent evaporation method. The two-component reversible thermochromic microcapsule provided by the disclosure can effectively alleviate the color lag phenomenon of a traditional three-component thermochromic capsule.