A composition includes two or more near infrared absorbing compounds having an absorption maximum in a wavelength range of 650 to 1000 nm and having a solubility of 0.1 mass % or lower in water at 23° C., in which the two or more near infrared absorbing compounds include a first near infrared absorbing compound having an absorption maximum in a wavelength range of 650 to 1000 nm, and a second near infrared absorbing compound having an absorption maximum in a wavelength range of 650 to 1000 nm which is shorter than the absorption maximum of the first near infrared absorbing compound, and a difference between the absorption maximum of the first near infrared absorbing compound and the absorption maximum of the second near infrared absorbing compound is 1 to 150 nm.

A fluorescent dye, a preparation method and uses thereof, wherein the fluorescent dye has the advantages of sensitivity and specificity to viscosity response, low background fluorescence and the like, and can also be used as a fluorescence activated and lightened-up probe for fluorescence labeling, quantification or detection of proteins, enzymes or nucleic acids.

Water-soluble dipyrromethene-based dyes are provided herein. In certain cases, the water-soluble dye is a dipyrromethene-boron dye. Embodiments of the subject water-soluble dipyrromethene-based dyes have a narrow absorption maxima in the yellow green range close to or at the 561 nm laser line, and are suitable for use in tandem dye systems. Embodiments of the subject dyes efficiently transfer energy to an acceptor chromophore while a second dipyrromethene-based dye of the same or different chemical structure is within energy-receiving proximity therewith. Also provided herein are tandem dyes that include the subject water-soluble dipyrromethene-based dyes, e.g., as donor chromophores. Methods of evaluating a sample for the presence of a target analyte and methods of labelling a target molecule in which the subject dyes find use are also provided. Systems and kits for practicing the subject methods are also provided.

The present invention relates to a method of visualizing biomolecules, having the steps of: a) providing a sample of immobilized biomolecules in a matrix and carry on the electrophoresis process; b) incubating the matrix of step a) in a solution containing a cyanine-derived molecule, for a time of 5 to 60 minutes, at room temperature, in a container preventing exposure to light, shaking the container at less of 75 rpm; c) transferring the matrix from step b) to a container with a solution having: at least one tetrazolium salt and incubating for a time of 15 to 120 minutes at room temperature under light exposure; d) removing the matrix with immobilized biomolecules from the previous step and washing with distilled water; and e) visualizing directly by the naked eye the biomolecules immobilized in the matrix.

Provided are a ruthenium complex dye having a water content of 0.2% to 4.0% by mass; a dye solution including the ruthenium complex dye; a photoelectric conversion element having semiconductor fine particles having the ruthenium complex dye carried thereon; and a dye-sensitized solar cell including the photoelectric conversion element.

There is provided a light absorption filter, an optical filter, an OLED display device, or a liquid crystal display device, containing a resin; a dye that has a main absorption wavelength band at a wavelength of 400 to 700 nm; and a compound that generates a radical upon ultraviolet irradiation, in which the dye includes a specific squarine-based coloring agent represented by General Formula (1) or a specific benzylidene-based coloring agent or cinnamylidene-based coloring agent represented by General formula (V).

Provided are fluorescent nanoparticles and their conjugates and methods of using the same for in vivo and in vitro diagnostics and other applications. In some embodiments, provided are fluorescent nanoparticles with high solid-state absolute quantum yield. In some embodiments, provided are methods of manufacturing such nanoparticles. Nanoparticles may comprise monomers, such as styrene, and fluorophores, such as AlEgen™ Bright Green.

Disclosed is a novel compound represented by formula (1) below. In the formula, A represents an optionally substituted aromatic hydrocarbon ring or aromatic heterocyclic group, B represents a group including a chain of one to four pieces of one or more groups selected from groups represented by specific formulae (B-1) to (B-13) (such as —C═C— or —N═N—, specifically see the description), R1 to R3 each represent an optionally substituted hydrocarbon or hydrocarbonoxy group, at least one of R1 to R3 represents an optionally substituted hydrocarbonoxy group, R4 and R5 each represent an optionally substituted hydrocarbon group, R4 and R5 may be linked together to form a ring, and R4 and R5 may be each independently linked with A to form a ring ##STR00001##

Voltage sensitive dyes comprising boron and related compositions and methods are provided. In some embodiments, a voltage sensitive dye comprises an electron acceptor comprising boron. The electron acceptor may be attached (e.g., covalently) to at least one electron donating group and at least one polar group. For instance, the electron acceptor may comprise optionally substituted boron dipyrromethene (e.g., optionally substituted 4,4-difluoro-4-bora-3a,4a-diaza-s-indacene). The point of attachment and chemical nature of the electron donating group(s) and polar group(s) may be selected to impart beneficial properties to the voltage sensitive dye. For instance, the voltage sensitive dye may have an extended difference in the dipole moment between the ground and electronic states due at least in part to the position of the electron donating group(s). The voltage sensitive dyes, described herein, may have high specificity, high signal to noise ratio, fast responsivity, high voltage sensitivity, high photostability, and/or high brightness.

The present invention relates to fluorescent dyes in general. The present invention provides a wide range of fluorescent dyes and kits containing the same, which are applicable for labeling a variety of biomolecules, cells and microorganisms. The present invention also provides various methods of using the fluorescent dyes for research and development, forensic identification, environmental studies, diagnosis, prognosis, and/or treatment of disease conditions.