A compound of Formula (I) or a pharmaceutically acceptable ester, amide, solvate, or salt thereof, or a salt of such an ester or amide or a solvate of such an ester amide or salt, is disclosed. Compositions comprising the compound and methods of use are also disclosed. Those dimeric Evans Blue derivatives, denoted as N(tEB)2, reversibly bind two molecules of albumin via the two albumin binding regions of each NtEB in the dimer, resulting in significantly increased binding affinity to albumin and extended circulation half-life in vivo. Further, when the N(tEB)2 is conjugated to a peptide therapeutic, the in situ formation of the complex of N(tEB)2 with two albumin molecules resulted in increased resistance of the peptide therapeutic from proteolysis.

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The invention relates to the use of pigment mixtures which contain at least one pigment of formulas (la) and (lb), wherein variables X.sup.1, X.sup.2, X.sup.3, X.sup.4, Y.sup.1, Y.sup.2, Z.sup.1 and Z.sup.2 are as ##STR00001##
defined and wherein the pigment mixtures are useful as dyes in printing methods.

The invention relates to the use of pigment mixtures which contain at least one pigment of formulas (la) and (lb), wherein variables X.sup.1, X.sup.2, X.sup.3, X.sup.4, Y.sup.1, Y.sup.2, Z.sup.1 and Z.sup.2 are as ##STR00001##
defined and wherein the pigment mixtures are useful as dyes in printing methods.

Compositions, methods, and kits for stable Trypan Blue solutions are provided. In one example, a method includes: cooling a solution of Trypan Blue; and filtering the cooled solution. In another example, a method includes: mixing a water-soluble polymer with a Trypan Blue solution. The method may further include adding one or more ingredients such as an aqueous buffer, an osmolyte, an acid, a base, a buffer, a cell culture medium, water, or combinations thereof.

Compositions, methods, and kits for stable Trypan Blue solutions are provided. In one example, a method includes: cooling a solution of Trypan Blue; and filtering the cooled solution. In another example, a method includes: mixing a water-soluble polymer with a Trypan Blue solution. The method may further include adding one or more ingredients such as an aqueous buffer, an osmolyte, an acid, a base, a buffer, a cell culture medium, water, or combinations thereof.

The invention relates to the use of pigment mixtures which contain at least one pigment of formulas (Ia) and (Ib), wherein, independent from each other, X1, X3 represent carbamoyl, C1-C4-alkylcarbamoyl, di(C1-C4)-alkylcarbamoyl, sulfamoyl, phenylsulfamoyl, C1-C4-alkylsulfamoyl or di(C1-C4)-alkylsulfamoyl; X2, X4 represents hydrogen, C1-C4-alkyl, C1-C4-alkoxy, halogen or nitro; Y1, Y2 represents hydrogen, halogen or C1-C4-alkyl, C1-C4-alkoxy or C1-C4-alkoxycarbonyl; and Z1, Z2 represents hydrogen, phenyl, naphthyl, benzimidazolonyl, substituted phenyl or substituted naphthyl, wherein the substituents can be 1, 2, 3 or 4 and selected from the groups halogen, nitro, cyano, C1-C4-alkoxycarbonyl, carbamoyl, C1-C4-alkylcarbamoyl, di(C1-C4)-alkylcarbamoyl, phenylcarbamoyl, sulfamoyl, phenylsulfamoyl, C1-C4-alkylsulfamoyl, di(C1-C4)-alkylsulfamoyl, C1-C4-acylamino, C1-C4-alkyl and C1-C4-alkoxy, and the pigments of formulas (Ia) and (Ib) are different. Said pigment mixtures are used as a dye in printing methods.

The invention relates to the use of pigment mixtures which contain at least one pigment of formulas (Ia) and (Ib), wherein, independent from each other, X1, X3 represent carbamoyl, C1-C4-alkylcarbamoyl, di(C1-C4)-alkylcarbamoyl, sulfamoyl, phenylsulfamoyl, C1-C4-alkylsulfamoyl or di(C1-C4)-alkylsulfamoyl; X2, X4 represents hydrogen, C1-C4-alkyl, C1-C4-alkoxy, halogen or nitro; Y1, Y2 represents hydrogen, halogen or C1-C4-alkyl, C1-C4-alkoxy or C1-C4-alkoxycarbonyl; and Z1, Z2 represents hydrogen, phenyl, naphthyl, benzimidazolonyl, substituted phenyl or substituted naphthyl, wherein the substituents can be 1, 2, 3 or 4 and selected from the groups halogen, nitro, cyano, C1-C4-alkoxycarbonyl, carbamoyl, C1-C4-alkylcarbamoyl, di(C1-C4)-alkylcarbamoyl, phenylcarbamoyl, sulfamoyl, phenylsulfamoyl, C1-C4-alkylsulfamoyl, di(C1-C4)-alkylsulfamoyl, C1-C4-acylamino, C1-C4-alkyl and C1-C4-alkoxy, and the pigments of formulas (Ia) and (Ib) are different. Said pigment mixtures are used as a dye in printing methods.

Compositions, methods, and kits for stable Trypan Blue solutions are provided. In one example, a method includes: cooling a solution of Trypan Blue; and filtering the cooled solution. In another example, a method includes: mixing a water-soluble polymer with a Trypan Blue solution. The method may further include adding one or more ingredients such as an aqueous buffer, an osmolyte, an acid, a base, a buffer, a cell culture medium, water, or combinations thereof.

Compositions, methods, and kits for stable Trypan Blue solutions are provided. In one example, a method includes: cooling a solution of Trypan Blue; and filtering the cooled solution. In another example, a method includes: mixing a water-soluble polymer with a Trypan Blue solution. The method may further include adding one or more ingredients such as an aqueous buffer, an osmolyte, an acid, a base, a buffer, a cell culture medium, water, or combinations thereof.