The invention refers to a method and a device for the phenotypic detection of carbapenemases and carbapenemase producers by adding a substrate of general formula A-(L)-M.sub.1-(X)—Z, where M.sub.1 is a carbapenem backbone, A or Z is a quencher, the other one of the two, Z or A, is a fluorophore, L is an optional linker, X is an optional leaving group for linking Z to the carbapenem backbone, and Z is an optional leaving group, to a sample suspected of containing such carbapenemase producers and/or carbapenenmases. The invention further refers to a method for the phenotypic detection of resistant bacteria, in particular 3MRGN or 4MRGN, by releasing the enzymes of a bacterial culture into a lysate during lysis and then subjecting the lysate, as the sample to be analyzed, to an aforementioned method in order to phenotypically detect the presence of resistance-conferring carbapenemases.

Graphene quantum dots are functionalized by covalently bonding a corrosion inhibitor molecule thereto. In a useful method, a corrosion inhibitor compound is blended with a graphene quantum dot-tagged corrosion inhibitor compound, and the blend is applied to a metal surface, such as the interior of a carbon steel pipe. The blend inhibits corrosion arising from contact with produced water generated by hydrocarbon recovery from one or more subterranean reservoirs. The produced water having the blend dispersed therein is irradiated with a source of light having a selected first range of wavelengths, and the luminescent emission of the graphene quantum dot-tagged corrosion inhibitor is measured at a selected second range of wavelengths, thereby providing for real-time measurement of corrosion inhibitor concentration within the pipe.

Graphene quantum dots are functionalized by covalently bonding a corrosion inhibitor molecule thereto. In a useful method, a corrosion inhibitor compound is blended with a graphene quantum dot-tagged corrosion inhibitor compound, and the blend is applied to a metal surface, such as the interior of a carbon steel pipe. The blend inhibits corrosion arising from contact with produced water generated by hydrocarbon recovery from one or more subterranean reservoirs. The produced water having the blend dispersed therein is irradiated with a source of light having a selected first range of wavelengths, and the luminescent emission of the graphene quantum dot-tagged corrosion inhibitor is measured at a selected second range of wavelengths, thereby providing for real-time measurement of corrosion inhibitor concentration within the pipe.

The present disclosure provides nanoparticle transducers and methods of use thereof for the detection of analyte concentrations in a fluid. Nanoparticle transducers can comprise a nanoparticle, such as a Pdot, coupled to an enzyme that catalyzes a reaction with the analyte. The nanoparticle transducers further comprise chromophores that emit fluorescence that varies as a function of the concentration of one of the elements of the reaction. The nanoparticle transducer thus changes fluorescence as the analyte concentration changes, transforming analyte concentration values into fluorescence intensities. The measurement of these intensities provides a measurement of the analyte concentration. The nanoparticle transducers are biocompatible, allowing for use in vivo, for the monitoring of analyte blood concentrations such as blood glucose concentrations.

The present disclosure provides nanoparticle transducers and methods of use thereof for the detection of analyte concentrations in a fluid. Nanoparticle transducers can comprise a nanoparticle, such as a Pdot, coupled to an enzyme that catalyzes a reaction with the analyte. The nanoparticle transducers further comprise chromophores that emit fluorescence that varies as a function of the concentration of one of the elements of the reaction. The nanoparticle transducer thus changes fluorescence as the analyte concentration changes, transforming analyte concentration values into fluorescence intensities. The measurement of these intensities provides a measurement of the analyte concentration. The nanoparticle transducers are biocompatible, allowing for use in vivo, for the monitoring of analyte blood concentrations such as blood glucose concentrations.

Provided herein are processes for preparing fluorescent 1-cyano-2-substituted isoindole compounds or N-substituted phthalazinium compounds, comprising reacting an aromatic dialdehyde or aromatic aldehyde-ketone compound with a material that contains primary amino or hydrazine groups, and assaying methods involving the processes thereof.

Provided herein are processes for preparing fluorescent 1-cyano-2-substituted isoindole compounds or N-substituted phthalazinium compounds, comprising reacting an aromatic dialdehyde or aromatic aldehyde-ketone compound with a material that contains primary amino or hydrazine groups, and assaying methods involving the processes thereof.

The present disclosure relates to the field of organic light emitting materials, and in particular, to a fluorescent probe compound for zinc ion, as well as a preparation method and use in zinc ion detection thereof. The fluorescent probe compound of the present disclosure has a name of 2-(7-(2,8-dimethyl quinoline-6-yl)-5,6,7,8-tetrahydro-1,8-naphthyridin-2-yl) phenol, and is synthesized with 2,8-dimethyl tetrahydroquinoline and 2-(2-phenolyl)-1,8-naphthyridine as main raw materials. Fluorescence property tests show that the fluorescent probe compound of the present disclosure has a high selectivity and sensitivity for Zn.sup.2+, a high chemical stability and a good water solubility, which particularly suitable for detecting Zn.sup.2+ in a water environment system. The excitation and emission spectrums of the compound are in a visible region, which could serve as a fluorescent probe applied to the field of zinc ion detection.

The present disclosure relates to the field of organic light emitting materials, and in particular, to a fluorescent probe compound for zinc ion, as well as a preparation method and use in zinc ion detection thereof. The fluorescent probe compound of the present disclosure has a name of 2-(7-(2,8-dimethyl quinoline-6-yl)-5,6,7,8-tetrahydro-1,8-naphthyridin-2-yl) phenol, and is synthesized with 2,8-dimethyl tetrahydroquinoline and 2-(2-phenolyl)-1,8-naphthyridine as main raw materials. Fluorescence property tests show that the fluorescent probe compound of the present disclosure has a high selectivity and sensitivity for Zn.sup.2+, a high chemical stability and a good water solubility, which particularly suitable for detecting Zn.sup.2+ in a water environment system. The excitation and emission spectrums of the compound are in a visible region, which could serve as a fluorescent probe applied to the field of zinc ion detection.

Described herein are 1,2-dioxetanes that are useful as chemiluminescent probes, diagnostic agents, and imaging agents. Also described herein are compositions containing such compounds and methods of using the same.