An ultrasonic-assisted heat press machine, includes a frame, a material tank, an extruder, an ultrasonic wave generator, and a heater. The material tank is disposed on the frame and includes a cavity for accommodating an oil-bearing raw material. The extruder is extended in the cavity. The ultrasonic wave generator is disposed on the frame and adjacent to the material tank. The heater is disposed on the frame and adjacent to the material tank. The heater is configured to produce heat to heat the cavity and the ultrasonic wave generator, and regulate the working frequency of the ultrasonic wave generator. When in use, the heater, the extruder, and the ultrasonic wave generator cooperate to separate oil from the oil-bearing raw material in the material tank.

The present invention relates to methods of synthesizing long-chain polyunsaturated fatty acids, especially eicosapentaenoic acid, docosapentaenoic acid and docosahexaenoic acid, in recombinant cells such as yeast or plant cells. Also provided are recombinant cells or plants which produce long-chain polyunsaturated fatty acids. Furthermore, the present invention relates to a group of new enzymes which possess desaturase or elongase activity that can be used in methods of synthesizing long-chain polyunsaturated fatty acids.

A solvent extraction process for extracting oil containing dihomolinolenic acid from marine macro-algae, since as ascophyllum. Harvested ascophyllum is desalted to a salt content of less than 3% by weight, and dried to a moisture content of less than 5% by weight, and is then chopped into pieces of maximum dimension not exceeding 5 mm. The desalted, dried and chopped ascophyllum is then packed into a cellulose soxhlet thimble (13) and covered with a silica glass wool. The soxhlet thimble (13) is then placed in a vessel (12) of a soxhlet apparatus (11). A solvent reservoir (15) is charged with the solvent, namely, a food grade hexane, and is evaporated from the solvent reservoir (15) and condensed in a condenser (22) above the vessel (12) and is drip-fed into the soxhlet thimble (13) for extracting the oil containing the dihomolinolenic acid from the ascophyllum. The solvent with the extracted oil entrained therein is returned to the solvent reservoir (15) and the process continues until substantially all the oil has been extracted from the ascophyllum. The oil containing the dihomolinolenic acid is then recovered from the solvent by low pressure distillation until the solvent content of the oil containing the dihomolinolenic acid has been reduced to less than 5% by weight. The oil containing the dihomolinolenic acid is then desolvated to remove the remaining solvent therefrom.

A method for recovering fish oil from aquatic biomass under cold conditions, the method comprising the steps of; providing an aquatic biomass; producing a minced aquatic biomass by mincing the aquatic biomass; providing an aqueous suspension of the minced aquatic biomass by mixing and/or homogenizing the minced aquatic biomass in an aqueous solution; adjusting the pH of said aqueous suspension to an extreme high pH or an extreme low pH; separating the aqueous suspension into a supernatant comprising a lower density emulsion fraction substantially comprising oil, aqueous solution and emulsified proteins, and a higher density fraction comprising substantially solubilized proteins, and optionally a pellet comprising collagenous components; collecting the lower density emulsion fraction; separating the lower density emulsion fraction into an oil phase and an aqueous phase; and collecting the oil from said oil phase.

A method for recovering fish oil from aquatic biomass under cold conditions, the method comprising the steps of; providing an aquatic biomass; producing a minced aquatic biomass by mincing the aquatic biomass; providing an aqueous suspension of the minced aquatic biomass by mixing and/or homogenizing the minced aquatic biomass in an aqueous solution; adjusting the pH of said aqueous suspension to an extreme high pH or an extreme low pH; separating the aqueous suspension into a supernatant comprising a lower density emulsion fraction substantially comprising oil, aqueous solution and emulsified proteins, and a higher density fraction comprising substantially solubilized proteins, and optionally a pellet comprising collagenous components; collecting the lower density emulsion fraction; separating the lower density emulsion fraction into an oil phase and an aqueous phase; and collecting the oil from said oil phase.

Provided are an oleaginous microorganism disruption process using a supersonic disperser and a method for producing bio-oil using the same. The method for producing bio-oil according to the present invention induces a cell disruption of oleaginous microorganisms without a separate drying process, thereby providing a method for continuously producing bio-oil in an economical and simple manner. In addition, the method of the present invention induces a cell disruption of oleaginous microorganisms without a heating process, thereby producing bio-oil without a change in physical properties due to the heat.

Provided are an oleaginous microorganism disruption process using a supersonic disperser and a method for producing bio-oil using the same. The method for producing bio-oil according to the present invention induces a cell disruption of oleaginous microorganisms without a separate drying process, thereby providing a method for continuously producing bio-oil in an economical and simple manner. In addition, the method of the present invention induces a cell disruption of oleaginous microorganisms without a heating process, thereby producing bio-oil without a change in physical properties due to the heat.

A method of extracting oil from biological raw material includes creating a slurry of biological raw material, raising or lowering a pH of the slurry to separate lipid and protein components in the slurry, further separating the lipid and protein components into a first lipid rich phase and a protein rich phase, adjusting a pH of the first lipid rich phase to a point at which additional proteins in the first lipid rich phase coagulate, and recovering a second lipid rich phase from the additional coagulated proteins.

A method designed to clarify the coffee oil contained in coffee grounds or in whole and/or damaged coffee beans. The method objective is achieved by starting with inoculation of the coffee grounds or coffee beans with macromycetes especially with white rot fungi, continuing with an incubation, step that allows complete population of the coffee grounds or coffee beans by the fungal mycelium to be achieved, and finishing with steps of drying and extracting the coffee oil. The method disclosed allows colourless or pale yellow coffee oil to be produced, favouring the use thereof in cosmetic and food products, amongst others.

The invention provides novel and improved methods that allow effective capture of valuable active ingredients in biomass such as whole stillage or thin stillage at cost-effective commercial scale. The invention also provides novel compositions of active ingredients with unique properties (e.g., nutritional values and enhanced bioavailability).