Ultrasonic devices comprise a housing comprising an ultrasonic surface, an ultrasonic transducer, and an ultrasonic horn to provide and focus energy from the ultrasonic transducer to the ultrasonic surface. Methods of removing trapped air bubbles from a cell culture container comprise positioning a cell culture container comprising air bubbles trapped in liquid within microcavity wells of the cell culture container at an ultrasonic surface of an ultrasonic device. Mechanical agitation is generated by the ultrasonic device and applied to the cell culture container to remove the trapped air bubbles from the microcavity wells. Methods of releasing cell aggregates from a cell culture container comprising positioning a cell culture container comprising cell aggregates in microcavity wells at an ultrasonic surface of an ultrasonic device. The cell aggregates are released from the microcavity wells by applying mechanical agitation from the ultrasonic device to a surface of the cell culture container.

A bioreactor has a container, an end plate, an agitator, a drive unit, and at least one field device arranged in the interior, field device electronics, a data transmission unit arranged on the end plate, and a motor head piece coupled in terms of energy to the drive unit. At least one communication unit is coupled in terms of signaling to the motor head piece and includes an adapter piece, having adapter electronics which are electrically connected to the field device electronics of the field device, and a communicator piece. The adapter piece and the communicator piece are separate components which can be coupled to one another, wherein the motor head piece is a separate component which can be coupled to the communication units.

Provided herein are systems and methods for thermally separating a flask and the base of an automatic flask stirring device. A thermal separator apparatus may be provided with a stand and a plurality of first standoffs. The stand may have a top surface configured to support a flask containing a substance to be stirred by a flask stirrer. The first standoffs may be located between the stand and a flask stirrer base, thereby creating a first air gap between the stand and the flask stirrer base. In some embodiments, the first air gap allows air to flow straight through the first air gap in a first direction.

A cell culture device is provided. A cell culture device according to an exemplary embodiment of the present invention includes: a main body comprising a culture space having one surface that is open; and a surface-modified sheet-type culture plate which is fixed to the main body to cover the opened one surface of the culture space, and thus forms a culture surface on which cells are cultured, and which forms the culture surface so that the cells can be attached to the culture surface.

Improved cell culture devices and related methods that overcome the limitations of prior devices and methods, by creating devices that can integrate a variety of novel attributes. These various attributes include the use of gas permeable material and medium volumes that exceed conventional devices as well as compartments that can facilitate the long term study of high density cultures with reduced disruption of the culture environment, the ability to study the migration of items of interest including substances such as chemokine, track the movement of cells, and monitor cell to cell interactions.

A system for processing samples from a body of fluid. The system includes one or more sample bottles for acquiring a sample from the body of fluid. Each sample bottle initially retains a pre-filling fluid. Each sample bottle includes a fluidic inlet port and a bottle outlet port. Each sample bottle has an inlet check valve coupled to the fluidic inlet port, the inlet check valve configured to allow fluid from the body of fluid into a sample bottle via the fluidic inlet port when the pressure difference between the body of fluid and fluid within the sample bottle reaches a threshold. The system further includes at least one pump, the bottle outlet port of each sample bottle selectively coupled to the at least one pump via a different control valve. The at least one pump is configured, in a first configuration, to remove prefilling fluid from each selected sample bottle such that, for each selected sample bottle, the pressure difference threshold is reached and a sample from the body of fluid is acquired.

A system for processing samples from a body of fluid. The system includes one or more sample bottles for acquiring a sample from the body of fluid. Each sample bottle initially retains a pre-filling fluid. Each sample bottle includes a fluidic inlet port and a bottle outlet port. Each sample bottle has an inlet check valve coupled to the fluidic inlet port, the inlet check valve configured to allow fluid from the body of fluid into a sample bottle via the fluidic inlet port when the pressure difference between the body of fluid and fluid within the sample bottle reaches a threshold. The system further includes at least one pump, the bottle outlet port of each sample bottle selectively coupled to the at least one pump via a different control valve. The at least one pump is configured, in a first configuration, to remove prefilling fluid from each selected sample bottle such that, for each selected sample bottle, the pressure difference threshold is reached and a sample from the body of fluid is acquired.

This disclosure provides a cell culture media extending material capable of releasing nutrients into the cell culture environment slowly overtime. In embodiments, this material is a part of a cell culture vessel. In embodiments, the material is a coating or a film on a surface of a cell culture material. In additional embodiments, the material is a surface upon which cells are cultured, such as a cell culture vessel or a microcarrier.

Herein is reported a bioreactor comprising a cultivation vessel and a reactor head plate, wherein the cultivation vessel has a working volume of from 20 ml to 350 ml and comprises two or more in-situ sensors, wherein the reactor head plate comprises an in-situ sensor port, wherein to the in-situ sensor port at least one in-situ glucose sensor and one in-situ pH sensor are connected.

The present disclosure relates generally to an apparatus and methods for microorganism isolation, characterization and identification based on oxygen, pressure, culture media gradients and metabolites. In each embodiment, the apparatus of the disclosure is particularly useful for the purpose of novel isolation of never before cultured species of microorganisms and their by-products.