Portable real-time airborne fungi acquiring and detecting equipment and method are provided, the equipment includes a light source device, a manual constant-flow air pump, an impactor, an airborne fungi enrichment and dyeing device, and a fluorescence data collecting and processing device sequentially connected. The fluorescence detection technology is combined with the microparticle separation technology to develop the portable airborne fungi real-time acquiring and detecting equipment. This equipment improves the complex and extensive collection methods in conventional airborne fungi detection and the demand limitation of independent detection equipment, and realizes the real-time collection and quantification of airborne fungi concentration. Moreover, the equipment has the advantages of small volume, low costs, easy operation and is easy to be prompted.

Portable real-time airborne fungi acquiring and detecting equipment and method are provided, the equipment includes a light source device, a manual constant-flow air pump, an impactor, an airborne fungi enrichment and dyeing device, and a fluorescence data collecting and processing device sequentially connected. The fluorescence detection technology is combined with the microparticle separation technology to develop the portable airborne fungi real-time acquiring and detecting equipment. This equipment improves the complex and extensive collection methods in conventional airborne fungi detection and the demand limitation of independent detection equipment, and realizes the real-time collection and quantification of airborne fungi concentration. Moreover, the equipment has the advantages of small volume, low costs, easy operation and is easy to be prompted.

Air-sampling devices for obtaining airborne microbial samples from an environment are provided. A weathershield is also described which may be used to shield an airsampling device from the weather, moisture, animals, and other potential contaminants. These components may be provided as part of an air-sampling kit.

A portable device for collecting particles and microorganisms is provided. The device includes a cyclone enclosure for centrifuging air, external air inlet for admitting air into the enclosure, and air outlet connected by a coupling to an air inlet filter of an individual motorized respiratory protection appliance.

A system for detecting airborne pathogens includes a pathogen collector having a surface area for engaging with and collecting pathogens; an electrochemical sensor for detecting pathogens on the pathogen collector, the electrochemical sensor comprising an electrode and a reactive substance selected to electrochemically react with the pathogens; and an air-flow generating pump. The system is configured such that the generated air flow moves pathogens in ambient air towards and/or along the surface area of the pathogen collector such that pathogens engage and accumulate on its surface area. The system is further configured such that the reactive substance of the electrochemical sensor electrochemically reacts with the accumulated pathogens. In response to the electrochemical reaction, the electrochemical sensor's electrode detects an electronic signal that indicates the presence of the pathogens.