Devices and methods to measure cells and/or tissue's contractile force are disclosed. Included is a mount with rigid, and non-rigid posts sized to flex. Determined is force exerted by contractile cells and tissues in a multiwell plate. The device is designed to fit inside individual wells with posts directed downwards. Posts are attached to a 3D printed circular mount with tabs for depth within the well. The mount has a window for medium changes while the device is positioned inside the well. The cells are seeded within a hydrogel. As the hydrogel condenses, cells/tissue wrap around the post's outside pulling non-rigid post toward rigid post. Inverted light microscope is used to determine deflection of non-rigid post inside the multiwell plate. Movement of the non-rigid post is measured using an acrylic ruler on an underside of the multiwell plate. Contractile forces of cells/tissue are determined using cantilever mechanics.

Provided is a test apparatus in which a test for bacterial identification or antimicrobial susceptibility can be promptly determined. A division state of bacteria is monitored by performing microscopic observation of shapes and the number of the bacteria in each of wells in a culture plate for bacterial identification culture or an antimicrobial susceptibility test, and it is determined whether or not the bacteria grow in a stage shifted from an induction phase to a logarithmic phase, with reference to an image obtained through microscopic observation. In addition, determination performed based on turbidity in the related art may be combined with determination performed based on microscopic observation in which change and the like in the shapes of the bacteria are monitored. Accordingly, it is possible to realize a highly accurate test result.

The invention is related to a device (1) for receiving a biological sample (3) wherein the device (1) comprises a plurality of wells (10) wherein each well (10) comprises an inner surface (14) facing a volume (60) for receiving a biological sample (3). The inner surface (14) comprises a top section (20) and a bottom section (30). The top section (20) and the bottom section (30) are connected via a circumferential step (40). The circumferential step (40) forms a stop for a tip of a pipette (70).

The present disclosure relates to a well plate for 3D cell spheroid culture that facilitates 3D cell spheroid culture and enables cell adhesion and detachment by adjusting a surface roughness, a method for manufacturing the well plate for 3D cell spheroid culture, and a method for 3D cell spheroid culture using the same.

The present invention relates to a microtissue compartment device, comprising a compartment structure (1) having an upper surface (2) and a lower surface (3) essentially coplanar thereto, and at least two wells (4) suitable for accommodating one or more microtissues (5) in a liquid volume, each well having a lower section (4a) with a given diameter, coaxially oriented thereto an upper section (4b) with an extended diameter, and at least one conduit (6) fluidically connecting at least two wells to one another, and at least one space (13) arranged above a well. At least one well has, in its upper section, a relief structure (9) that prevents spreading or overflow of a liquid volume comprised in said well into space (13).

A sensing cell culture vessel having one or more sensors on or in the vessel is configured to collect readings of various parameters or characteristics of a cell culture located within the sensing cell culture vessel and transmit the readings. The sensing cell culture vessels may be accompanied by a sensing plate having means for reading the one or more sensors and transmitting the one or more sensors to a server hosting electronic lab notebook for analyzing and storage of the readings. Sensing plates may further be equipped with cameras for imaging cell cultures located in the sensing cell culture vessels and transmitting the images to the server hosting the electronic lab notebook for analyzing and storage of the images. Embodiments of the invention allow for the continuous and automatic monitoring of cell cultures.

A spheroid cell culture article including: a frame having a chamber including: an opaque side wall surface; a top aperture; a gas-permeable, transparent bottom; and optionally a chamber annex surface and second bottom, and at least a portion of the transparent bottom includes at least one concave arcuate surface, is disclosed. Methods of making and using the article are also disclosed.

An apparatus, that relates to the field of in vitro fertilization, is provided for the combined incubation and vitrification of a biological material. The apparatus can be configured to allow for automatic incubation and vitrification of a viable biological material. Thereby predetermined protocols for handling the biological material can be performed precisely and accurately thus avoiding errors and deviations from the intended protocol, as caused by manual human intervention.

[Object] To provide a mixing device capable of accurately mixing a solution in a multi-well plate.

[Solving Means] A mixing device 1 is configured to be attachable to a multi-well plate 30 and includes a casing 100, a plurality of stirrers 11, a plurality of motors 12 as a drive portion, and a mounting portion 16. The casing 100 includes a main surface portion 101 facing an upper surface 301 of the multi-well plate 30. The stirrers 11 protrude from the main surface portion 101 toward wells 31 of the multi-well plate 30. The motors 12 are disposed to the casing 100 and rotate the stirrers 11 about axes thereof. The mounting portion 16 is provided to the casing 100 and is mounted to the multi-well plate 30 to position the casing 100 on the multi-well plate 30.

The invention provides a technology for promptly determining bacterial identification or an antimicrobial susceptibility testing. In the invention, first, a state where the bacteria are divided is monitored by performing microscopic observation with respect to the shape or the number of bacteria in each of wells of a culture plate for bacterial identification culture or the antimicrobial susceptibility testing. In addition, the shape, the number or the area of the bacteria are interpreted from the image obtained by the microscopic observation whether or not the bacteria proliferate at a stage from an induction phase to a logarithmic phase, and the time-dependent changes thereof are made into a graph. From the graph, it is determined whether or not the bacteria proliferate for each measurement, the determination results are displayed on the screen, and accordingly, the result of the antimicrobial susceptibility is provided every time when the measurement is performed (FIG. 12).