A cell culture insert with a hollow cylindrical housing having an upper end-face opening delimited by an opening edge and a lower end-face base designed as a membrane, support feet being arranged on the base edge of the housing and/or on the base and at least one support arm. The legs protrude downward for supporting the housing on a support in a non-tipping manner with a small uniform spacing between the base and the support. The at least one support arm protrudes outwards at the opening edge and can be placed on an edge of a well plate in which a plurality of wells. The support arm is an edge hanging element, and for this purpose, has a support section that runs radially relative to the housing and a hanging section that runs from the support section downwards in the direction of the base of the housing.

A new pumpless internal circulation photobioreactor for hydrogen production, including: an outer reaction barrel and an inner reaction barrel made of transparent materials, a gas collecting device, and an air duct arranged between the inner reaction barrel and the outer reaction barrel. An outer ring-shaped reaction chamber is formed between the inner reaction barrel and the outer reaction barrel. Liquid permeation holes and air holes are arranged on the inner reaction barrel. The gas collecting device is communicated with the outer ring-shaped reaction chamber. The air duct is communicated with the interior of the inner reaction barrel and the outer ring-shaped reaction chamber. The present invention has a simple and compact structure and is easy to operate, and gas produced by the reactor can be re-introduced into the reaction solution to provide aerodynamic power for stirring the reaction solution, which realizes low energy consumption and high-efficiency hydrogen production.

A device for growing microorganisms. The device can include a self-supporting, water-proof substrate having an inner-facing surface and an outer-facing surface; a cover sheet having an inner-facing surface and an outer-facing surface, the cover sheet adhered to at least a portion of the substrate; a microstructured surface on the inner-facing surface of the coversheet, wherein the microstructured surface comprises a plurality of microstructures; a substantially dry, gelling agent and a substantially dry, microbial growth nutrient composition disposed on the microstructured surface of the microstructured film or on a portion of the inner-facing surface of the substrate.

A cell culture device is provided. A cell culture device according to an exemplary embodiment of the present invention includes: a main body comprising a culture space having one surface that is open; and a surface-modified sheet-type culture plate which is fixed to the main body to cover the opened one surface of the culture space, and thus forms a culture surface on which cells are cultured, and which forms the culture surface so that the cells can be attached to the culture surface.

An apparatus suitable for handling biological material, for directly introducing or removing material to, or from, a container, comprising a body comprising at least one resealable port; a cover comprising an aperture and wherein the cover is disposed over an upper side of the body, and wherein the cover is moveable such that an aperture of the cover is configured to expose at least part of the resealable port.

The invention generally relates to containers for cell and tissue culturing with multiple compartments in fluid communication with each other to provide a common culture environment in each of the compartments while maintaining physical separation of cells and tissue therein. The invention further relates to culture containers providing a sterile culture environment with detachably coupleable lids and open access to each compartment within a container.

The invention discloses a bioreactor with a vessel defining an inner volume, agitation means and at least one addition tube, wherein a delivery orifice in the addition tube is located within the inner volume and a check valve is arranged in proximity of the delivery orifice for allowing flow of a fluid in the direction from the addition tube into the inner volume of the vessel and blocking flow in the reverse direction.

A cell culture chamber device for growing cell cultures and tissues. The device includes: an enclosure containing a cell culture media, the enclosure being defined partly by a first end, a second end, and a connecting wall. The first end or a part or window thereof is substantially transparent, and the second end and/or the connecting wall, or a respective part or window thereof, is/are substantially transparent/translucent. The first end is configured to be optically aligned, at least for some period of time or periodically, with the second end and/or with the connecting wall so that light or another illumination or visualisation signal, transmitted through or by the second end and/or through or by the connecting wall into the enclosure is transmitted through the cell culture media and out through the first end to outside the enclosure, and e.g. to outside the cell culture chamber device.

Herein is reported a bioreactor comprising a cultivation vessel and a reactor head plate, wherein the cultivation vessel has a working volume of from 20 ml to 350 ml and comprises two or more in-situ sensors, wherein the reactor head plate comprises an in-situ sensor port, wherein to the in-situ sensor port at least one in-situ glucose sensor and one in-situ pH sensor are connected.

A culture system for co-culturing a first cell group consisting of one or more kinds of cells and a cell layer or tissue formed of a second cell group consisting of one or more kinds of cells different from the former cells comprising: a first culture tank for co-culturing under anaerobic conditions the first cell group consisting of one or more kinds of cells and the cell layer or tissue formed of the second cell group consisting of one or more kinds of cells; a second culture tank for pooling a liquid culture medium of aerobic conditions; one or more substance-exchange structures that are disposed so as to connect the first culture tank to the second culture tank; and the aforesaid cell layer or tissue that is disposed so as to cover the surface on the first culture tank side of the substance-exchange structure(s).