A cell culture apparatus includes a flow passage in which cell suspension containing at least one of cells or cell masses as granular bodies is to flow, and an imaging unit that is provided in a middle of the flow passage and continuously images the plurality of granular bodies contained in the cell suspension to acquire a plurality of images while the cell suspension flows in the flow passage.

Containment and facility systems for offshore porous floating bioreactors that contain algae water slurries in saltwater environments. A containment system may include a containment boom system, a containment silt curtain system, a containment marineworks system, or a combination thereof. Facility systems include an offshore porous floating bioreactor in fluid communication with at least an onshore algae harvesting system. Algae cultivation may occur at an onshore algae cultivation system or within a porous floating bioreactor, and algae lipid production occurs within a porous floating bioreactor. The lipid induced algae is received by the onshore algae harvesting system for further processing.

A method of transferring a liquid between multiple containers in cell culture. A first container of multiple culture containers each having a port for transferring content, a three-way stopcock and a medium supply container having one port are connected by a tube, and one pump is arranged therebetween; when the number of containers in the multiple culture containers is two, the three-way stopcock and a second culture container are connected by a tube; when the number is three or more, a three-way stopcock is newly provided between the immediately connected culture container and the three-way stopcock, and connection of the newly provided three-way stopcock and a subsequent culture container by a tube is repeated, whereby all culture containers are connected to the three-way stopcock; and the culture medium is transferred from the medium supply container to each container in the multiple culture containers via the three-way stopcock by the pump.

This invention is a system for the production of biomass from photosynthesizing microorganisms that includes a photobioreactor comprising a transparent panel made from two transparent sheets with a separation between them, with top and bottom openings and with transparent, parallel subdivisions that form a panel of vertically arranged, transparent cells, where each transparent cell has a top opening and a bottom opening; a lower recirculation chamber in fluid contact with the bottom openings of the transparent panel; an upper recirculation chamber in fluid contact with the top openings of the transparent panel; a gas distribution tube externally arranged along the edge of said transparent panel; where said gas distribution tube comprises gas injectors arranged in fluid contact with the interior of a plurality of transparent cells; and a supporting structure that supports the transparent panel, the lower recirculation chamber, the upper recirculation chamber and the air distribution tube.

According to present disclosure, there is disclosed an algae growth and cultivation system that provides a cost-efficient means of producing algae biomass as feedstock for algae-based products, such as, fertilizer, feed, biofuel manufacture, and desirably impacts, nutrient recovery from waste streams for valued byproducts production, recycle water, and alternative/renewable energy production. The system as discussed herein is an integrated systems approach to wastewater treatment, algal strains selection for byproducts production, and recycle of algal biomass-processing waste or additional algae harvested as feedstock for products such as fertilizer production. Embodiments of a system as discussed herein present an economically viable algae production system and process that allows algae-derived products such as fertilizer, feed, biofuels, etc. to compete with non-organic or petroleum products in the marketplace.

A multi-chamber single-use bioreactor for cell culture expansion has bag assembly and a rigid support structure defining a bag receiving space. The bag assembly disposed in the bag receiving space of the rigid support structure and supported by the rigid support structure. The bag assembly has at least a first flexible bag and a second flexible bag. The first bag defines a first reaction chamber, and the second bag defines a second reaction chamber. The first reaction chamber has a first volume, a first inlet, and a first outlet, and the second reaction chamber has a second volume different from the first volume, a second inlet, and a second outlet. The second inlet of the second bag is fluidically connected to the first outlet of the first bag so liquid in first reaction chamber can be transferred to the second reaction chamber.

A bioprocess system and a method for incubating, growing and harvesting cell cultures is described. Also disclosed is a bioprocess container that can be used with the system. In one aspect of the present disclosure, the bioprocess system includes bioprocess tubes and cell culture tubes having particular dimensions and being made from specific materials that allow the tubes to be welded together while preventing open connections and/or ruptures. In this manner, bioprocess containers can be connected and disconnected from a cell culture apparatus without having to perform the manipulation within a closed environment and without associated monitoring.

Methods for the continuous production, capturing and purification of biologics such as recombinant proteins are described. Also described are pharmaceutical compositions comprising such biologics, as well as methods of treatment and uses of such biologics.

There is disclosed a modular anaerobic digestion point-of-waste to renewable energy system. The system is directed to a modular and scalable anaerobic digestion system for point-of-waste use. The System includes a pretreatment process for removing inhibitory nutrients from a feedstock, an in-treatment process for providing clean renewable energy and a post-treatment process for further providing clean renewable energy for subsequent use. The System includes a leaching bed; a liquids tank; a mixing tank; an anaerobic digester reactor; a precipitation tank; a stripping tank; a hydrogen sulfide scrubber; a water remover; a gas bladder; a dewaterer; and a flare system.

A system and method for the production of microbial consortiums and by-product material is provided. A physical containment system comprising phase spaces arranged in a discrete order to favor specific biological reactions is also provided. Phase profiles and phase data sets include the pre-determined physical and biological parameters for the phase space transitions. Movement of material from one phase to the next is hydraulically balanced enabling working fluid to continuously move in a fixed direction and rate of flow. Continuous monitoring of phase profiles and phase data sets provide feedback to the system enabling alteration of the conditions in the system to control reactions therein.