The present disclosure relates to a well plate for 3D cell spheroid culture that facilitates 3D cell spheroid culture and enables cell adhesion and detachment by adjusting a surface roughness, a method for manufacturing the well plate for 3D cell spheroid culture, and a method for 3D cell spheroid culture using the same.

A spheroid cell culture article including: a frame having a chamber including: an opaque side wall surface; a top aperture; a gas-permeable, transparent bottom; and optionally a chamber annex surface and second bottom, and at least a portion of the transparent bottom includes at least one concave arcuate surface, is disclosed. Methods of making and using the article are also disclosed.

A bioreactor system is provided that includes a cell culture vessel having a first end, a second end, and at least one reservoir between the first and second ends; and a cell culture matrix disposed in the at least one reservoir. The cell culture matrix has a structurally defined substrate with a surface for adhering cells thereto. The bioreactor system flows material through the at least one reservoir and through the cell culture matrix in a flow direction from the first end to the second end, and the cell culture matrix exhibits isotropic fluid flow permeability therethrough.

A microwell perfusion plate system includes a plate and at least one well on the plate. Each well includes: a porous membrane; a through-pore microwell membrane above and on the porous membrane, the microwell membrane including a plurality of microwells with a respective microwell configured to hold a 3D cell culture, wherein a respective microwell includes a top opening and a bottom opening; an inlet passageway in fluid communication with each top opening of the plurality of microwells and configured to deliver liquid medium to the plurality of microwells and the 3D cell cultures held therein; an outlet passageway in fluid communication with each bottom opening of the plurality of microwells and configured to receive the liquid medium from the plurality of microwells; and a cell culture well directly above the microwell membrane, wherein the cell culture well defines at least a portion of the inlet passageway.

A cell culture insert with a hollow cylindrical housing having an upper end-face opening delimited by an opening edge and a lower end-face base designed as a membrane, support feet being arranged on the base edge of the housing and/or on the base and at least one support arm. The legs protrude downward for supporting the housing on a support in a non-tipping manner with a small uniform spacing between the base and the support. The at least one support arm protrudes outwards at the opening edge and can be placed on an edge of a well plate in which a plurality of wells. The support arm is an edge hanging element, and for this purpose, has a support section that runs radially relative to the housing and a hanging section that runs from the support section downwards in the direction of the base of the housing.

A cell culture device is provided. A cell culture device according to an exemplary embodiment of the present invention includes: a main body comprising a culture space having one surface that is open; and a surface-modified sheet-type culture plate which is fixed to the main body to cover the opened one surface of the culture space, and thus forms a culture surface on which cells are cultured, and which forms the culture surface so that the cells can be attached to the culture surface.

A system and method for studying cell injury mechanisms by applying biologically relevant mechanical impact to in vitro cell culture are disclosed. This approach is for maintaining consistent in vitro conditions during experiments, accommodating multiple cell populations, and monitoring each in real-time while achieving amplitude and time scale of input acceleration that mimic blunt injury cases. These multiplexed, environmental control capabilities enable characterizing the relationships between mechanical impact and cell injury in multivariate biological systems.

The present disclosure concerns a cell or tissue culture system comprising a solid support for the culture of adherent cells or adherent tissues and a plurality of cationic dendrimers associated to the surface of the solid support. Each cationic dendrimer includes one or more functional amine group. The cationic dendrimer is protonated at physiological pH. The cell or tissue culture system can be used for the culture of adherent cells or tissues and be used for the differentiation of stem cells.

The object is achieved by an organic-inorganic composite dispersion liquid comprising organic-inorganic composite particles (X) which are formed of a polymer (P) of a monomer including a (meth)acrylamide-based monomer (a) and one or more inorganic materials (B) selected from a water-swellable clay mineral or silica in a three-dimensional network and are dispersed in an aqueous medium (C), in which an average particle diameter of the organic-inorganic composite particles (X) is 100 nm or more.

A transfection device suitable for delivery of various macrostructures (e.g., mitochondria, bacteria, liposomes) is described and uses mechanical force to thereby induce active endocytosis in a target cell. Contemplated devices are able to achieve high throughput of transfected cells that remain viable and are capable of producing colonies.