The present invention relates to a reactor comprising a vessel (1) for containing: • a mass to be treated, and • at least one lighting device (2a, 2b) intended to promote the treatment of said mass, characterized in that each lighting device (2a, 2b) comprises a light diffuser including at least one micro-etched plate (211) which is transparent to light radiation.

A cell culture method is a cell culture method for arranging cultured cells in a culture dish and continuously culturing the cultured cells by supplying a liquid required to grow or maintain the cultured cells to the culture dish and discharging the liquid from the culture dish. The cell culture method includes: providing a supply port of the liquid at one end of the culture dish and providing a discharge port of the liquid at other end of the culture dish so as to sandwich the cultured cells between the supply port and the discharge port, and discharging the liquid while supplying the liquid to the culture dish so that a moving linear velocity of the liquid from the supply port toward the discharge port is less than a maximum velocity at which shear stress is not applied to the cultured cells.

A sun-tracking light distributor system for use in an open-ended photo-bioreactor having an aqueous liquid for a photosynthetic culture, comprising: at least one light distributor each including a concentrator supporting section with a light entry surface adapted to receive sunlight rays, an elongated rod section with a light distribution surface adapted to redirect the received sunlight rays within the aqueous liquid, a light concentrating element provided at the light entry surface which concentrates within the elongated rod at least a portion of the sunlight rays received at the light entry surface; a displacement system operatively connected to the light distributors and adapted to change an orientation of the light entry surface of the light distributors to track a solar position.

An incubator and shelf with integrated microscope and wireless transmitter comprises a shelf adapted for use inside an incubator; a microscope integrated into the shelf; and a wireless (such as Wi-Fi) transmitter that wirelessly transmits an image produced by the microscope. Embodiments may be powered by the incubator, and may have an inverted microscope that views an object from below.

This disclosure provides systems and methods for integrating an array of electronic sensors capable of performing trans-epithelial electrical resistance (TEER) measurements into a microfluidic device that includes a well plate. In some implementations, the sensors can include electrodes that are submerged into fluidically connected wells of the microfluidic device, which can contain an electrically conductive fluid such as the cell culture media or a buffered salt solution. An array of such electrodes can be integrated into a lid of the system that includes the microfluidic device. These electrodes can be routed using a printed circuit board through a number of multiplex switches that can allow addressing of a desired unit of the device through a microprocessor in communication with a computer.

A multi-layer culture vessel observation system, a cart device, and a multi-layer culture vessel observation device that enable an operator to easily observe objects to be observed in a multi-layer culture vessel are provided. A multi-layer culture vessel observation system includes: a cart device 20 capable of carrying a multi-layer culture vessel 30 that includes a plurality of trays and moving; and an observation device 10 capable of observing objects to be observed in each tray in the multi-layer culture vessel 30, wherein the cart device 20 includes a frame body including a sideways exposure part that exposes two side faces of the multi-layer culture vessel 30 from an upper end to a lower end, wherein the observation device 10 includes: a storage 14 that stores the cart device 20 in a state of carrying the multi-layer culture vessel 30; and an imaging device 11 that includes an optical system and outputs an image formed by the optical system, and wherein, when the cart device 20 carrying the multi-layer culture vessel 30 is stored in the storage 14, the sideways exposure part is located on an optical axis of the imaging device 11.

Techniques are described for automated microinjection of substances, such as genetic material, into single cells in tissue samples. An example system comprises a robotic manipulator apparatus configured to hold and position a micropipette. Furthermore, the system comprises a microscope camera positioned to observe an injection site. A computing device receives image data from a microscope camera of the system, where the image data represents an image of a tissue sample. The computing device receives, via a user interface, an indication of a line traced by a user on the image of a tissue sample. In response, the computing device controls the robotic manipulator apparatus to move a tip of the micropipette along a path defined by the trajectory line. The pressure controller injects a gas into the micropipette to eject a substance out of the micropipette at one or more points along the path defined by the trajectory line.

Techniques are described for automated microinjection of substances, such as genetic material, into single cells in tissue samples. An example system comprises a robotic manipulator apparatus configured to hold and position a micropipette. Furthermore, the system comprises a microscope camera positioned to observe an injection site. A computing device receives image data from a microscope camera of the system, where the image data represents an image of a tissue sample. The computing device receives, via a user interface, an indication of a line traced by a user on the image of a tissue sample. In response, the computing device controls the robotic manipulator apparatus to move a tip of the micropipette along a path defined by the trajectory line. The pressure controller injects a gas into the micropipette to eject a substance out of the micropipette at one or more points along the path defined by the trajectory line.

The present disclosure provides modules, instruments and methods to enrich for cells edited via nucleic acid-guided nuclease editing of live cells.

The present disclosure provides modules, instruments and methods to enrich for cells edited via nucleic acid-guided nuclease editing of live cells.