An example apparatus comprises a thermal cell lysis chamber, including a substrate and a lid coupled to the substrate to form a microfluidic channel therethrough. The apparatus includes cell detection circuitry to detect presence of a cell within the microfluidic channel and to detect lysis of the cell. The apparatus also includes a thermal lysing element disposed in the lid to apply heat to a cell detected by the cell detection circuitry, and lysis control circuitry. The lysis control circuitry is to regulate a temperature applied by the thermal lysing element, based on detection by the cell detection circuitry of a cell within the microfluidic channel and based on detection by the cell detection circuitry of a lysis event, and record the temperature applied by the thermal lysing element at which the lysis event occurred.

The invention relates to a method for fractionating the components of a biomass of protein-rich microalgae of the genus Chlorella, characterized in that it comprises the following steps: providing a microalgal biomass produced by fermentation, optionally, washing the biomass so as to eliminate the interstitial soluble compounds, thermal permeabilization of the biomass at a temperature of between 50 and 150° C., preferably 100 and 150° C., for a duration of between 10 seconds and 5 minutes, preferably for a duration of between 5 seconds and 1 minute, separation between the biomass thus permeabilized and the soluble fraction by a centrifugation technique, more particularly multistage centrifugation, optionally, recovery and clarification of the soluble fraction obtained in this way by microfiltration so as to remove residual insoluble substances therefrom, separation of the preceding soluble fraction by precipitation, so as to obtain a peptide isolate and a peptide concentrate.

[Problem] It is an object of the present invention to provide a method for collecting seawater that contains plankton and producing DHMBA, which is an antioxidant, from the plankton contained in the seawater.

[Solution] The method of the present invention includes: filtering collected seawater containing the plankton using a filter; taking out a cell content from the plankton remaining on the filter; subsequently heating/pressurizing the cell content taken out; and producing 3,5-dihydroxy-4-miethoxybenzyl alcohol from the heated/pressurized product. The plankton is a diatom. [Selected Drawing] FIG. 1

The present invention relates to a method for separating larvae into a pulp fraction and a liquid fraction, including the steps of introducing living larvae into a grinding apparatus whist adding water, grinding the larvae by means of counter-rotating screws and separating the ground biomass of larvae into a pulp and liquid fraction. In particular, the invention is applicable to the larvae of the black soldier fly and produces a chitin-rich pulp and a fat-and-protein-rich liquid fraction.

The present disclosure is in the field of ‘pharmacognosy’ and ‘chemistry of natural products’. The present disclosure generally relates to a process of isolation and purification of Biochemical Constituents from algae. The present disclosure particularly relates to a process of isolation and purification of Biochemical Constituents from a biomass of cyanobacteria. The present disclosure provides a process for isolating and extracting phycocyanins, chlorophylls, proteins and polysaccharides from the spirulina biomass.

The present invention provides a production method for a composition for cell culturing. This production method comprises: (1) a step for subjecting algae to an acid hydrolysis treatment and/or an alkali hydrolysis treatment; (2) a step for neutralizing the hydrolysis product obtained in the step (1) to obtain an algae extract; and (3) a step for mixing the algae extract with a medium for cell culturing, wherein the medium for cell culturing does not substantially contain L-glutamine.

The present invention relates to a method for obtaining yeast proteins comprising the following steps: a) providing a yeast cream; b) exposing this yeast cream to a thermal plasmolysis at a temperature between 70 and 95° C. for a period between 30 seconds and 4 hours, preferably between 1 minute and 3 hours, more preferably between 40 minutes and 2 hours; b′) separating the insoluble fraction and the soluble fraction; c) subjecting the insoluble fraction to the activity of at least one ribonuclease and a glucanase, sequentially or simultaneously, at a temperature between 40 and 65° C., preferably 60° C., for a period between 8 and 24 hours, preferably 18 hours; d) separating the insoluble fraction from the soluble fraction; wherein the insoluble fraction collected in step d) has no taste, having a nucleotide content less than 3% and a true protein content of at least 72%. Step b′) is optional. In this case, the entirety of the composition obtained after thermal plasmolysis of the yeast cream is subjected to enzymatic activity.

Oscillating angularly rotating a container containing a material may cause the material to be separate. Denser or heavier material may unexpectedly tend to collected relatively close to the axis of rotation, while less dense or light material may tend to collect relatively away from the axis of rotation. Oscillation along an arcuate path provides high lysing efficiency. Alternatively, a micromotor may drive an impeller removably received in a container. Lysing may be implemented in batch mode, flow-through stop or semi-batch mode, or flow-through continuous mode. Lysing particulate material may exceed material to be lysed or lysed material and/or air may be essentially eliminated from a chamber to increase lysing efficiency.

A method for producing biomass from a microalgae includes culturing the microalgae in an effluent diluted in seawater. A method for bioremediating an effluent includes culturing a microalgae in the effluent diluted in seawater. The microalgae is at least one of a strain of the genus Nodularia, a strain of the genus Chrysoreinhardia, a strain of the genus Halochlorella, or combinations thereof. At the beginning of culturing, the diluted effluent exhibits concentrations of total nitrogen (N) in the range of 30-150 mg/l and concentrations of total phosphorus (P) in the range of 1-15 mg/l. The N/P quotient is in the range of 5-40.

The present invention relates to a method for extracting nucleic acids from a biological sample, and the extraction method presents a novel method for effectively extracting nucleic acids. When nucleic acids are extracted from biological samples in the related art, various impurities present in the biological samples are not properly removed, such that the purification rate is low, but the present invention provides a method for extracting nucleic acids from a biological sample of which the bacteria, virus and nucleic acid recovery rates are enhanced, by adding a surfactant and a sodium sulfate (Na.sub.2SO.sub.4) solution in a biological sample disruption step and a purification step, thereby enabling pathogens to be detected more sensitively and accurately.