The present invention relates to RNA-free mammal serums which can be useful for cell culture or for producing pharmaco-biological products, due to the fact that they maintain their supplement features. Another embodiment of the invention refers to the method for removing RNA from mammal serum through the application of sequential serum heating, alkalisation and neutralisation steps.

The present invention relates to RNA-free mammal serums which can be useful for cell culture or for producing pharmaco-biological products, due to the fact that they maintain their supplement features. Another embodiment of the invention refers to the method for removing RNA from mammal serum through the application of sequential serum heating, alkalisation and neutralisation steps.

The invention relates to the use of a protein having nuclease activity for reducing the viscosity and/or preventing an increase in viscosity of a fermentation broth, which comprises intact microorganisms after termination of the fermentation process. The invention moreover relates to a method for reducing the viscosity and/or preventing an increase in viscosity of a fermentation broth comprising intact microorganisms after fermentation and comprising a step of introducing a protein having nuclease activity into the fermentation broth during the fermentation until the start of recovering the end product. The invention further relates to fermentation processes comprising said use of a protein having nuclease activity to allow more flexibility to the downstream processing of the fermentation broth.

The present disclosure is generally related to compositions and methods for constructing and obtaining Bacillus sp. host cells (strains) having enhanced protein production phenotypes. Certain embodiments of the disclosure are therefore related to genetically modified Bacillus sp. cells derived (obtained) from parental Bacillus sp. cells (e.g., such as parental Bacillus cells expressing/producing a protein of interest). Thus, certain embodiments are directed to modified Bacillus sp. cells expressing/producing an endogenous protein of interest, or a heterologous protein of interest, wherein the modified Bacillus sp. cell comprises a genetic modification of a yitMOP operon, a combined genetic modification of a yitMOP operon and a sdpABC operon, a genetic modification of a yitM gene and the like, wherein the modified Bacillus sp. cells produce an increased amount of the POI relative the parental cells from which they were derived (i.e., when grown/cultivated/fermented under identical conditions).

The present disclosure is generally related to compositions and methods for constructing and obtaining Bacillus sp. host cells (strains) having enhanced protein production phenotypes. Certain embodiments of the disclosure are therefore related to genetically modified Bacillus sp. cells derived (obtained) from parental Bacillus sp. cells (e.g., such as parental Bacillus cells expressing/producing a protein of interest). Thus, certain embodiments are directed to modified Bacillus sp. cells expressing/producing an endogenous protein of interest, or a heterologous protein of interest, wherein the modified Bacillus sp. cell comprises a genetic modification of a yitMOP operon, a combined genetic modification of a yitMOP operon and a sdpABC operon, a genetic modification of a yitM gene and the like, wherein the modified Bacillus sp. cells produce an increased amount of the POI relative the parental cells from which they were derived (i.e., when grown/cultivated/fermented under identical conditions).

The objective of the present invention is to provide a method for effectively and easily reducing an amount of a specific impurity in a liquid. The method for reducing an amount of a nucleic acid in a liquid according to the present invention is characterized in comprising the steps of contacting the liquid with a water-insoluble magnesium compound to adsorb at least a part of the nucleic acid on the water-insoluble magnesium compound. Also, the objective of the present invention is to provide an adsorbing filter useful for purifying a useful substance, such as an antibody and an antibody-like molecule, used as a purification material for effectively removing an impurity with easily maintaining the yield of the target substance due to excellent adsorption ability to a nucleic acid and low adsorption ability to an antibody, an antibody-like molecule or the like. The adsorbing filter is characterized in comprising the layer comprising a water-insoluble magnesium compound.

The objective of the present invention is to provide a method for effectively and easily reducing an amount of a specific impurity in a liquid. The method for reducing an amount of a nucleic acid in a liquid according to the present invention is characterized in comprising the steps of contacting the liquid with a water-insoluble magnesium compound to adsorb at least a part of the nucleic acid on the water-insoluble magnesium compound. Also, the objective of the present invention is to provide an adsorbing filter useful for purifying a useful substance, such as an antibody and an antibody-like molecule, used as a purification material for effectively removing an impurity with easily maintaining the yield of the target substance due to excellent adsorption ability to a nucleic acid and low adsorption ability to an antibody, an antibody-like molecule or the like. The adsorbing filter is characterized in comprising the layer comprising a water-insoluble magnesium compound.

The present disclosure is generally directed to an anucleated cell-based platforms for encapsulation and delivery of agricultural compounds. Disclosed herein are compositions for the stable and targeted delivery of agricultural compounds within achromosomal and/or anucleated cells. The present disclosure also provides methods of improving encapsulation and retention of agricultural compounds in achromosomal and/or anucleated cells.

The present disclosure is generally directed to an anucleated cell-based platforms for encapsulation and delivery of agricultural compounds. Disclosed herein are compositions for the stable and targeted delivery of agricultural compounds within achromosomal and/or anucleated cells. The present disclosure also provides methods of improving encapsulation and retention of agricultural compounds in achromosomal and/or anucleated cells.

The present invention relates to a method for degrading DNA in a sample obtained by microbial fermentation or bio-transformation, comprising treating the sample with a combination of increased temperature and low pH. It also relates to a method for releasing DNA from a microbial cell, comprising incubating the microbial cell at a temperature of 45° C. to 55° C. for two to ten hours. Finally, the present invention provides a method for producing a product, comprising a step of releasing DNA from a microbial cell and degrading said DNA.