CRISPR-based gene-drive system for inhibiting antibiotic resistance of bacteria, including Escherichia coli that efficiently copies a gRNA cassette and adjacent cargo that are flanked with sequences homologous to the targeted gRNA/Cas9 cleavage site. This “pro-active” genetic system (Pro-AG) functionally inactivates an antibiotic resistance marker on a high copy number plasmid with greater efficiency than control CRISPR-based methods. Pro-AG can effectively edit large plasmids or single-copy genomic targets, or introduce functional genes, with numerous applications to biotechnology and biomedicine.

CRISPR-based gene-drive system for inhibiting antibiotic resistance of bacteria, including Escherichia coli that efficiently copies a gRNA cassette and adjacent cargo that are flanked with sequences homologous to the targeted gRNA/Cas9 cleavage site. This “pro-active” genetic system (Pro-AG) functionally inactivates an antibiotic resistance marker on a high copy number plasmid with greater efficiency than control CRISPR-based methods. Pro-AG can effectively edit large plasmids or single-copy genomic targets, or introduce functional genes, with numerous applications to biotechnology and biomedicine.

The invention provide a system for locating an object in a volume of space, comprising: a communication device (CD) of the object disposed in the volume of space, wherein the communication device comprises a CD radio frequency (RF) receiver, a CD RF transmitter, and a CD infrared (IR) receiver; a first electrical device of a plurality of electrical devices disposed in the volume of space, wherein the first electrical device comprises at least one first sensor, a first RF receiver, a first IR transmitter, and a first RF transmitter, wherein the first RF transmitter broadcasts a first communication signal, wherein the first IR transmitter broadcasts a second communication signal, and wherein the first RF receiver receives, in response to the first communication signal received by the CD RF receiver and the second communication signal received by the CD IR receiver, a third communication signal broadcast by the CD RF transmitter of the communication device; and a controller communicably coupled to the plurality of electrical devices, wherein the controller receives a fourth communication signal sent by the first RF transmitter of the first electrical device, wherein the fourth communication signal comprises a first identification of the object and a second identification of the first electrical device, wherein the fourth communication signal is associated with a signal strength of the third communication signal received by the first RF receiver of the first electrical device.

The invention provide a system for locating an object in a volume of space, comprising: a communication device (CD) of the object disposed in the volume of space, wherein the communication device comprises a CD radio frequency (RF) receiver, a CD RF transmitter, and a CD infrared (IR) receiver; a first electrical device of a plurality of electrical devices disposed in the volume of space, wherein the first electrical device comprises at least one first sensor, a first RF receiver, a first IR transmitter, and a first RF transmitter, wherein the first RF transmitter broadcasts a first communication signal, wherein the first IR transmitter broadcasts a second communication signal, and wherein the first RF receiver receives, in response to the first communication signal received by the CD RF receiver and the second communication signal received by the CD IR receiver, a third communication signal broadcast by the CD RF transmitter of the communication device; and a controller communicably coupled to the plurality of electrical devices, wherein the controller receives a fourth communication signal sent by the first RF transmitter of the first electrical device, wherein the fourth communication signal comprises a first identification of the object and a second identification of the first electrical device, wherein the fourth communication signal is associated with a signal strength of the third communication signal received by the first RF receiver of the first electrical device.

Provided is a mutant strain having improved aromatic amino acid production capability as a result of inactivation or weakening of activity of glutaminase which is expressed by glutaminase B (glsB) gene.

Provided is a mutant strain having improved aromatic amino acid production capability as a result of inactivation or weakening of activity of glutaminase which is expressed by glutaminase B (glsB) gene.

Systems and kits are disclosed herein for genetic engineering (such as for DNA cleavage and gene-editing), which include catalytic nucleic acids and catalytic nucleic acid-assisting reagents. Methods of genetic engineering are also described, in which both catalytic nucleic acid-assisting reagents and catalytic nucleic acids are specific for a target site, thus, providing high-fidelity genetic engineering.

Provided are a 4-ureido-5-carboxyl-imidazole-amide hydrolase and use thereof, particularly use in the treatment of gout.

Provided are a 4-ureido-5-carboxyl-imidazole-amide hydrolase and use thereof, particularly use in the treatment of gout.

This invention pertains to optimized protein fusion linkers for creating multi-functional chimeric proteins and methods of using the same. Additionally, the invention pertains to chimeric proteins for use in guided endonuclease systems.