Embodiments of the present disclosure are directed to a method that includes contacting a population of plant cells with a messenger ribonucleic acid (mRNA) construct including a sequence encoding a rare-cutting endonuclease and a detectable label, wherein the rare-cutting endonuclease is configured to induce a mutation at a target genomic locus. The method further includes screening the population of plant cells for the detectable label to identify target plant cells that are genetically transformed with the mRNA construct.

Compositions and methods for transforming cells and generating transgenic grapevine plants comprising two silencing suppressor proteins C2 and V2 encoded by GRBV and recombinant hairpin vectors targeting C2 and V2, which are be used to generate stably transformed transgenic grapevine plants.

This disclosure provides an engineered system comprising: a first nucleic acid molecule encoding a CasX nuclease, and a guide RNA (gRNA) or a second nucleic acid molecule encoding the gRNA, where the first nucleic acid molecule is codon optimized for a eukaryotic cell, and where the gRNA is designed to hybridize with a target site in the eukaryotic cell. Further, this disclosure provides a method of modifying at least one target site in a eukaryotic genome comprising: providing a eukaryotic cell with a CasX nuclease or a first nucleic acid molecule encoding the CasX nuclease, and providing the eukaryotic cell with a guide RNA (gRNA) or a second nucleic acid molecule encoding the gRNA, where the gRNA and the CasX nuclease form a complex, where the gRNA hybridizes to the target site, and where the complex generates a modification at the target site.

The present invention provides a tobacco plant which is suitable for cultivation for harvesting leaf tobaccos. The present invention includes (i) a tobacco plant in which a mutation for suppressing the development of axillary buds is introduced and (ii) a method of producing the tobacco plant.

Genome editing including the introducing of precise gene edits is well established in diploid plants. Methods well established in the art introduce double strand DNA breaks in the genome of a plant applying technologies such as Zn-finger nucleases, homing endonucleases, TALEN or RNA guided nuclease e.g. Cas9 or Cas12a.

Described in certain exemplary embodiments herein are programmable DNA nuclease systems and/or components thereof that include or are otherwise associated with a ligase. Also described in certain exemplary embodiments herein are method of using the DNA nuclease systems described herein to modify a nucleic acid sequence.

The present invention discloses a method for selective crop management in real time. The method comprises steps of: (a) producing a biosensor plant, said biosensor plant comprises a visual biomarker, said biomarker is encoded by at least one modified genetic locus comprising (i) preselected reporter gene allele having a phenotype detectable by a sensor, and (ii) a regulatory region of a preselected gene allele responsive to at least one parameter or condition of said plant or its environment, said regulatory region is operably linked to said reporter gene, such that the expression of said reporter gene phenotype is correlated with the status of said at least one parameter or condition of said biosensor plant or its environment; (b) acquiring image data of a target area comprising a plurality of said biosensor plants via said sensor and processing said data to generate a signal indicative of the phenotypic expression of said reporter gene allele of said biosensor plant; and (c) communicating said signal to an execution unit communicably linked to the sensor, said execution unit is capable of exerting in real time a selective monitoring and/or treatment of said target area or a portion thereof comprising said biosensor plants, said treatment is being responsive to said status of said parameter or condition of the biosensor plant or its environment. The present invention further discloses systems and plants related to the aforementioned method.

Constructs for genetically engineering plants to selectively alter DELLA gene expression to promote plant growth while maintaining root integrity are provided, as are methods of designing, making and using such constructs.

A method of modifying a gene encoding or processed into a non-coding RNA molecule having no RNA silencing activity in a plant cell is disclosed. The method comprising introducing into the plant cell a DNA editing agent conferring a silencing specificity of the non-coding RNA molecule towards a target RNA of interest. A method of modifying a gene encoding or processed into a RNA silencing molecule in a plant cell is also disclosed. The method comprising introducing into the plant cell a DNA editing agent which redirects the silencing specificity of the non-coding RNA molecule towards a target RNA of interest. Plant cells, plant seeds, plants, and methods of generating plants are also disclosed.

TALEN compositions and methods of use are disclosed, which include using multiplexing compositions to create a targeted mutation in several genes at once, such as the FAD3 A/B/C genes, compositions to create a targeted mutation in a single gene, such as a gene encoding a FAD2 protein, and combinations thereof. The compositions and methods can provide gene-edited plants, plant parts, and plant cells that have improved characteristics compared to the corresponding unaltered plants, plant parts, or plant cells. For example, soybean plants, plant parts and plant cells that are capable of producing a seed with an oil having comparatively higher levels of oleic acid and lower levels of linoleic and linolenic acid than a corresponding seed lacking the targeted mutation are also provided.