The present disclosure relates to a well plate for 3D cell spheroid culture that facilitates 3D cell spheroid culture and enables cell adhesion and detachment by adjusting a surface roughness, a method for manufacturing the well plate for 3D cell spheroid culture, and a method for 3D cell spheroid culture using the same.

Engineered skin substitutes comprising an outer-facing portion and an inner-facing portion and methods of making the same are provided. The skin substitutes are configured to conform to a shape and a dimension of a body part of a subject, and have at least one surface that circles back on itself so as to enclose at least a portion of the body part. In some instances, dermis and epidermal layers can be formed in an air liquid interface. The exemplary skin substitutes are wearable and can be made to conform to a generic body part or a specific body part from a three-dimensional representation of the body part.

A culture manufacturing method that includes: bringing adherent cells into contact with a dissolvable culture carrier that is larger than the size of the adherent cells and disposing the adherent cells on the surface of the dissolvable culture carrier, subjecting the adherent cells disposed on the surface of the dissolvable culture carrier to suspension culture in a culture medium, subjecting the dissolvable culture carrier to a modification treatment that modifies at least a portion of the surface in order to detach the adherent cells in the suspension culture from the surface of the dissolvable culture carrier, and, following the modification treatment, separating and harvesting the adherent cells from the modified dissolvable culture carrier that is larger than the size of the adherent cells on the basis of the size difference.

The present disclosure aims to provide a manufacturing method of a cell structure. The manufacturing method comprises producing a coated region in which a culturing surface is coated with a temperature-responsive polymer or a temperature-responsive polymer composition, forming a droplet of a cell suspension in the coated region, and performing cell culturing in the droplet. A surface zeta potential of the coated region is 0 mV to 50 mV.

A cell culture substrate includes: a first layer that includes a first gel in which gold nanoparticles dispersed; and a second layer that includes a second gel in which the gold nanoparticles are not present or are present in a lower concentration in comparison with the first layer.

A method of preparing PNVCL block polymers as the substrate for thermo-sensitive cultureware is provided. A hydrophobic polymer of poly n-butyl mathacrylate (PBMA) is obtained by atom transfer radical polymerization (ATRP) with typical haloalkane as an initiator. Further a thermo-sensitive block copolymer of poly n-vinyl caprolactam (PNVCL) is obtained by polymerization of N-vinyl caprolactam (NVCL) monomers using the hydrophobic PBMA polymer as a macroinitiator.

A cell treatment agent containing alginate sulfate as an active ingredient, and a set reagent for activating suspended or dormant cells, which is a combination reagent of the cell treatment agent and an activator containing polyvalent cations are provided.

The present disclosure provides a substrate for cell culture. Systems comprising the substrate, and methods for using and manufacturing the substrate are also disclosed herein.

The purpose of the present invention is to provide a chondrocyte culture with high tissue regeneration ability. This purpose is met by a method involving a step in which a cell population separated from cartilage tissue is cultured on a thermoreversible polymer.

Provided herein is a method for making a tissue engineering scaffold. The method includes layering at least one sheet of cells onto a flexible scaffold, casting the sheets into geometries, and thereby creating the tissue engineering scaffold. Preferred geometry are non-linear (i.e. not a substantially flat surface such as may be provided by a flat glass substrate). The flexible scaffold is characterized by tensile strength, viscosity, stress, strain, modulus of polymers, or any combination thereof.