The present invention includes a seed, a plant, a protoplast, a hybrid and methods of making the same of a cotton cultivar recombinantly modified overexpresses at least one of AtRAV1, AtRAV2 to confer longer fibers to transgenic cotton plants under drought conditions without an effect on yield.

Simultaneous expression of a plurality of foreign genes by using a stealthy RNA gene expression system that is a complex that does not activate the innate immune mechanism and is formed from an RNA-dependent RNA polymerase, a single-strand RNA binding protein, and negative-sense single-strand RNAs including the following (1) to (8): (1) a target RNA sequence that codes for any protein or functional RNA; (2) an RNA sequence forming a noncoding region and derived from mRNA; (3) a transcription initiation signal sequence recognized by the RNA-dependent RNA polymerase; (4) a transcription termination signal sequence recognized by the polymerase; (5) an RNA sequence containing a replication origin recognized by the polymerase; (6) an RNA sequence that codes for the polymerase; (7) an RNA sequence that codes for a protein for regulating the activity of the polymerase; and (8) an RNA sequence that codes for the single-strand RNA binding protein.

The invention relates to a method for producing a recombinant protein in cells with a cell wall, comprising the step of increasing the secretion of the recombinant protein through the cell wall by expression of the protein in the cells in a culture medium containing a combination of a surface-active polymer and monovalent metal ions and with an osmolarity at least 0.32 osmol/L, said invention further relating to culture media and nutrient mixtures for the method.

The present invention relates to transgenic grape cells, compositions comprising such cells or extracts or fractions thereof, and methods of use thereof for inhibiting or reducing the incidence of cytokine release syndrome or cytokine storm in a subject. Disclosed herein are also methods for preventing, treating, reducing the incidence, suppressing or inhibiting a coronavirus infection or a symptom thereof.

The present invention relates to transgenic grape cells, compositions comprising such cells or extracts or fractions thereof, and methods of use thereof for inhibiting or reducing the incidence of cytokine release syndrome or cytokine storm in a subject. Disclosed herein are also methods for preventing, treating, reducing the incidence, suppressing or inhibiting a coronavirus infection or a symptom thereof.

The present invention relates to a method for introducing a polynucleotide into non-adhesively growing plant cells, comprising the following steps: providing a solid support having immobilized thereto the polynucleotide in dry state; contacting the plant cells with the polynucleotide on the solid support so as to obtain transformed plant cells; and optionally washing the plant cells.

The present invention relates to a method for introducing a polynucleotide into non-adhesively growing plant cells, comprising the following steps: providing a solid support having immobilized thereto the polynucleotide in dry state; contacting the plant cells with the polynucleotide on the solid support so as to obtain transformed plant cells; and optionally washing the plant cells.

The present invention provides a Chlorella variabilis-derived phosphomannose isomerase gene, herein named ChloPMI. The present invention also provides a prokaryotic expression vector comprising ChloPMI, which can be used for identifying mannose metabolic activity of ChloPMI protein. Further, the present invention provides an expression cassette and a plant expression vector comprising ChloPMI, and a use of the expression cassette and the expression vector in genetic transformation of plants. According to the present invention, the transformation of rice cells is successfully achieved with the plant expression vector constructed from the ChloPMI gene using mannose as a selection agent. According to the present invention, a plant-derived phosphomannose isomerase gene is successfully separated and cloned from Chlorella variabilis. Since the plant-derived phosphomannose isomerase gene is derived from Chlorella variabilis, it is environment-friendly and has no potential hazard to human, which is very beneficial in promoting and applying transgenic products and eliminating any existing doubts on transgenes.

The present invention provides a Chlorella variabilis-derived phosphomannose isomerase gene, herein named ChloPMI. The present invention also provides a prokaryotic expression vector comprising ChloPMI, which can be used for identifying mannose metabolic activity of ChloPMI protein. Further, the present invention provides an expression cassette and a plant expression vector comprising ChloPMI, and a use of the expression cassette and the expression vector in genetic transformation of plants. According to the present invention, the transformation of rice cells is successfully achieved with the plant expression vector constructed from the ChloPMI gene using mannose as a selection agent. According to the present invention, a plant-derived phosphomannose isomerase gene is successfully separated and cloned from Chlorella variabilis. Since the plant-derived phosphomannose isomerase gene is derived from Chlorella variabilis, it is environment-friendly and has no potential hazard to human, which is very beneficial in promoting and applying transgenic products and eliminating any existing doubts on transgenes.

The present disclosure describes a DNA fragment mixture for the prevention or for the treatment of at least one pathogenic, parasitic or infesting species of plants or of the environment, wherein the DNA mixture consists of random fragments of total DNA of at least one pathogenic, parasitic, or infesting species, and/or at least one phylogenetically similar species, against which the prevention and treatment are directed. Further, the disclosure describes a process and related system for improvement of the production/growth of microorganisms at high yield in bioreactors or photobioreactors, or of plants in different culture systems, where the nucleic acids of the organisms produced/grown by such a process are removed from the culture medium and the culture medium, deprived of these nucleic acid, is used again in the process.