Patent classifications
C12P13/10
PROCESS FOR ENRICHING THE BIOMASS OF MICROALGAE OF THE THRAUSTOCHYTRIUM GENUS WITH DHA AND WITH ARG AND GLU AMINO ACIDS
The present invention relates to a process for enriching a biomass of microalgae of the Thraustochytrium genus with DHA and with arginine and glutamic acid amino acids, characterized in that it comprises a step aimed at limiting the rate of growth of the microalga while at the same time maintaining or continuously introducing a source of nitrogen in or into the fermentation medium.
METHOD OF PRODUCING FLAVOR BY MIXED FERMENTATION OF HETEROLOGOUS MICROORGANISMS
The present invention relates to a method of producing a flavor by a co-fermentation process using mixed fermentation of two or more different microorganisms producing different products. The method of producing a flavor may produce a natural flavor capable of improving the taste and aroma of food and the overall sensory properties of food through a fermentation broth containing amino acids, nucleic acids and/or organic acids, which is produced by mixed fermentation of different microorganisms producing different products, that is, different kinds of amino acids, nucleic acids and/or organic acids. This flavor may be used in various food fields.
GENE ENGINEERING BACTERIA FOR PRODUCING L-ARGININE AND CONSTRUCTION METHOD AND APPLICATION OF GENE ENGINEERING BACTERIA
Disclosed are gene engineering bacteria for producing L-arginine and a construction method and an application of the gene engineering bacteria. According to the method, genes encoding a carbamoyl phosphate synthetase and a gene encoding an L-arginine biosynthesis pathway enzyme are integrated into Escherichia coli; the present invention has analyzed and reconstructed the arginine synthetic pathway and the metabolic flow related to arginine in the entire amino acid metabolic network in E. coli and finally obtained a genetically engineered bacterial strain which has a clear genetic background, carries no plasmids, undergoes no mutagenesis and is capable of stably and efficiently producing L-arginine.
GENE ENGINEERING BACTERIA FOR PRODUCING L-ARGININE AND CONSTRUCTION METHOD AND APPLICATION OF GENE ENGINEERING BACTERIA
Disclosed are gene engineering bacteria for producing L-arginine and a construction method and an application of the gene engineering bacteria. According to the method, genes encoding a carbamoyl phosphate synthetase and a gene encoding an L-arginine biosynthesis pathway enzyme are integrated into Escherichia coli; the present invention has analyzed and reconstructed the arginine synthetic pathway and the metabolic flow related to arginine in the entire amino acid metabolic network in E. coli and finally obtained a genetically engineered bacterial strain which has a clear genetic background, carries no plasmids, undergoes no mutagenesis and is capable of stably and efficiently producing L-arginine.
Modified polypeptide having an activity of ornithine-based product exporter and method for producing ornithine-based product using cells expressing the polypeptide
The present disclosure relates to a novel polypeptide having an ability to export an ornithine-based product, and a method for producing an ornithine-based product using the same.
Modified polypeptide having an activity of ornithine-based product exporter and method for producing ornithine-based product using cells expressing the polypeptide
The present disclosure relates to a novel polypeptide having an ability to export an ornithine-based product, and a method for producing an ornithine-based product using the same.
Method for the protein enrichment of microalgal biomass
The invention relates to a method for the protein enrichment of a heterotrophically cultured microalga, the microalga being of the genus Chlorella, even more particularly Chlorella protothecoides, characterized in that it comprises: a first step directed toward limiting the ammonium supply so as to obtain a microalgal biomass with a protein content of less than 50% expressed as N.6.25, preferably less than 30%, more preferentially between 20 and 25%; a second step in which the ammonium supply in the fermentation medium is increased so as to obtain a protein content of greater than 50%, preferably greater than 60%, more preferentially greater than 65%.
Method for the protein enrichment of microalgal biomass
The invention relates to a method for the protein enrichment of a heterotrophically cultured microalga, the microalga being of the genus Chlorella, even more particularly Chlorella protothecoides, characterized in that it comprises: a first step directed toward limiting the ammonium supply so as to obtain a microalgal biomass with a protein content of less than 50% expressed as N.6.25, preferably less than 30%, more preferentially between 20 and 25%; a second step in which the ammonium supply in the fermentation medium is increased so as to obtain a protein content of greater than 50%, preferably greater than 60%, more preferentially greater than 65%.
MICROORGANISM COMPRISING VARIANT LYSE AND METHOD OF L-AMINO ACID PRODUCTION USING SAME
Provided are a microorganism comprising variant LysE, and an L-amino acid producing method using same. The variant LysE may improve L-amino acid excretion and/or production capacity compared to a wild type.
MICROORGANISM COMPRISING VARIANT LYSE AND METHOD OF L-AMINO ACID PRODUCTION USING SAME
Provided are a microorganism comprising variant LysE, and an L-amino acid producing method using same. The variant LysE may improve L-amino acid excretion and/or production capacity compared to a wild type.