Provided is a method including extending a ssDNA by sequentially adding a plurality of modified nucleoside triphosphates to the ssDNA, wherein the base of the modified nucleoside triphosphates includes a primary modification selected from (i) a primary polynucleotide attached to the base of the modified nucleoside triphosphate, and (ii) a site on the base for covalent attachment of a primary polynucleotide to the base, further comprising covalently attaching a primary polynucleotide to the base after the polymerizing.

Provided is a method including extending a ssDNA by sequentially adding a plurality of modified nucleoside triphosphates to the ssDNA, wherein the base of the modified nucleoside triphosphates includes a primary modification selected from (i) a primary polynucleotide attached to the base of the modified nucleoside triphosphate, and (ii) a site on the base for covalent attachment of a primary polynucleotide to the base, further comprising covalently attaching a primary polynucleotide to the base after the polymerizing.

The present invention relates to a kit for detecting miRNA and a method for detecting miRNA using the kit. According to the miRNA detection kit and method of the present invention, it is possible to detect a certain miRNA in a quick and accurate manner, and it also possible to perform multiplex analysis capable of detecting a plurality of miRNAs at the same time.

The present invention relates to a kit for detecting miRNA and a method for detecting miRNA using the kit. According to the miRNA detection kit and method of the present invention, it is possible to detect a certain miRNA in a quick and accurate manner, and it also possible to perform multiplex analysis capable of detecting a plurality of miRNAs at the same time.

A method of detecting a ligase expressing micro-organism in a sample comprises steps of treating the sample under conditions that inhibit the activity of ATP-dependent ligase from mammalian cells but which do not inhibit the activity of the microbial ligases, contacting the sample or a portion of the sample with a nucleic acid molecule which acts as a substrate for ligase activity in the sample, incubating the thus contacted sample under conditions suitable for ligase activity; and specifically determining the presence and/or the amount of a ligated nucleic acid molecule resulting from the action of the ligase on the substrate nucleic acid molecule to indicate the presence of the ligase expressing micro-organism. The micro-organism may be a fungus or a bacterium or both. High pH conditions may be employed to inactivate mammalian ligases. Related kits are described.

The present disclosure relates to methods and apparatus for synthesising polynucleotides such as DNA and RNA in the absence of a template, to polynucleotides synthesised therefrom and to a kit of parts for synthesising polynucleotides.

The present disclosure relates to methods and apparatus for synthesising polynucleotides such as DNA and RNA in the absence of a template, to polynucleotides synthesised therefrom and to a kit of parts for synthesising polynucleotides.

Provided are methods and systems for encoding data into nucleic acid molecules. Methods and systems disclosed can include the use of promiscuous template nucleic acid molecules which enables data encoding using environmental modifications to yield encoded nucleic acid molecules.

A primer set includes a terminal primer A including, in its 3′-terminal part, a nucleotide sequence that hybridizes to a 3′-terminal part of a complementary sequence of a first target nucleic acid sequence, a k-th double-headed primer including two polynucleotides linked at their 5′ terminal sides, wherein one of the two polynucleotides includes, in its 3′-terminal part, a nucleotide sequence that hybridizes to a 3′-terminal part of a nucleotide sequence of a k-th target nucleic acid, and the other polynucleotide includes, in its 3′-terminal part, a nucleotide sequence that hybridizes to a 3′-terminal part of a complementary sequence of a (k+1)th target nucleic acid, and a terminal primer B including, in its 3′-terminal part, a nucleotide sequence that hybridizes to a 3′-terminal part of a nucleotide sequence of a N-th target nucleic acid.

A primer set includes a terminal primer A including, in its 3′-terminal part, a nucleotide sequence that hybridizes to a 3′-terminal part of a complementary sequence of a first target nucleic acid sequence, a k-th double-headed primer including two polynucleotides linked at their 5′ terminal sides, wherein one of the two polynucleotides includes, in its 3′-terminal part, a nucleotide sequence that hybridizes to a 3′-terminal part of a nucleotide sequence of a k-th target nucleic acid, and the other polynucleotide includes, in its 3′-terminal part, a nucleotide sequence that hybridizes to a 3′-terminal part of a complementary sequence of a (k+1)th target nucleic acid, and a terminal primer B including, in its 3′-terminal part, a nucleotide sequence that hybridizes to a 3′-terminal part of a nucleotide sequence of a N-th target nucleic acid.