Provided is a method for detecting and quantifying a nucleic acid in real time and at high speed. The present disclosure provides a real-time high-speed PCR method in which fluorescent signals can be measured from a single-wavelength light source by using a single signal fluorescent material under continuous temperature control. Thus, the PCR method can be performed with a compact lightweight device with a simplified structure.

Provided is a method for detecting and quantifying a nucleic acid in real time and at high speed. The present disclosure provides a real-time high-speed PCR method in which fluorescent signals can be measured from a single-wavelength light source by using a single signal fluorescent material under continuous temperature control. Thus, the PCR method can be performed with a compact lightweight device with a simplified structure.

A composition for binding to human papillomavirus type 16 (HPV16)-positive tumor cells, the composition including a DNA aptamer. The DNA aptamer includes one of SEQ ID NO: 1 and SEQ ID NO: 2.

The present disclose provides methods and systems for amplifying and quantifying nucleic acids and for detecting the presence or absence of a target in a sample. The methods and systems provided herein may utilize a device comprising a plurality of partitions separated from an external environment by a gas-permeable barrier. Certain methods disclosed herein involve subjecting nucleic acid molecules in the plurality of partitions to conditions sufficient to conduct nucleic acid amplification reactions. The nucleic acid molecules may be subjected to controlled heating in the plurality of partitions to generate data indicative of a melting point(s) of the nucleic acid molecules.

The present disclose provides methods and systems for amplifying and quantifying nucleic acids and for detecting the presence or absence of a target in a sample. The methods and systems provided herein may utilize a device comprising a plurality of partitions separated from an external environment by a gas-permeable barrier. Certain methods disclosed herein involve subjecting nucleic acid molecules in the plurality of partitions to conditions sufficient to conduct nucleic acid amplification reactions. The nucleic acid molecules may be subjected to controlled heating in the plurality of partitions to generate data indicative of a melting point(s) of the nucleic acid molecules.

The present disclosure provides for acoustofluidic centrifuge systems that can enrich and separate nanoparticles disposed in a fluid, such as liquid droplets, in a fast and efficient manner. Exemplary systems include a sound wave generator, such as a pair of slanted interdigitated transducers, and a containment boundary, such as a PDMS ring. The sound wave generator can produce surface acoustic waves that are capable of driving droplets to spin in a manner that can separate different sized particles into groups. In some embodiments, the acoustofluidic centrifuge system can include a plurality of containment boundaries in fluid communication with each other, allowing particles to separate between the containment boundaries. Methods of operating such systems, including methods of isolating different exosome subpopulations, are also disclosed.

The present disclosure provides for acoustofluidic centrifuge systems that can enrich and separate nanoparticles disposed in a fluid, such as liquid droplets, in a fast and efficient manner. Exemplary systems include a sound wave generator, such as a pair of slanted interdigitated transducers, and a containment boundary, such as a PDMS ring. The sound wave generator can produce surface acoustic waves that are capable of driving droplets to spin in a manner that can separate different sized particles into groups. In some embodiments, the acoustofluidic centrifuge system can include a plurality of containment boundaries in fluid communication with each other, allowing particles to separate between the containment boundaries. Methods of operating such systems, including methods of isolating different exosome subpopulations, are also disclosed.

Described herein are systems and methods for multiplexed analysis of two or more targets in a test sample including a first set of particles including a first set of target-specific reagents and a first optically detectable identifier capable of emitting a first wavelength indicative of a first target, and at least one second set of particles including a second set of target-specific reagents and a second optically detectable identifier capable of emitting a second wavelength indicative of a second target; and at least one optically detectable reporter probe capable of constitutively emitting a third wavelength in response to reaction of the first set of target-specific reagents with the first target in the test sample and/or reaction of the second set of target-specific reagents with the second target in the test sample, wherein the first wavelength, the second wavelength, and the third wavelength are optically discernable from one another.

Described herein are systems and methods for multiplexed analysis of two or more targets in a test sample including a first set of particles including a first set of target-specific reagents and a first optically detectable identifier capable of emitting a first wavelength indicative of a first target, and at least one second set of particles including a second set of target-specific reagents and a second optically detectable identifier capable of emitting a second wavelength indicative of a second target; and at least one optically detectable reporter probe capable of constitutively emitting a third wavelength in response to reaction of the first set of target-specific reagents with the first target in the test sample and/or reaction of the second set of target-specific reagents with the second target in the test sample, wherein the first wavelength, the second wavelength, and the third wavelength are optically discernable from one another.

The present disclosure relates, in part, to improved methods of making single-stranded DNA (ssDNA) from double-stranded DNA (dsDNA), as well as use of the resulting ssDNA for genome engineering. The disclosure also relates, in part, to improved methods of genetic modification using single stranded DNA binding proteins.