The present disclosure provides methods, compositions, and systems for distributing polymerase compositions into array regions. In particular, the described methods, compositions, and systems utilize density differentials and/or additives to increase efficiency in the distribution of polymerase compositions to a surface as compared to methods utilizing only diffusion control.

The present disclosure provides methods, compositions, and systems for distributing polymerase compositions into array regions. In particular, the described methods, compositions, and systems utilize density differentials and/or additives to increase efficiency in the distribution of polymerase compositions to a surface as compared to methods utilizing only diffusion control.

Various aspects of the present disclosure provide methods, apparatus and systems for single-molecule biosensors having nanowire or nanoribbon bridges between electrodes for sequencing and information storage and reading. In various embodiments, the present disclosure provides nanofabrication of biomolecular sensing devices beginning with parallel arrangements of transferable nanowires or nanoribbons, and provides in general methods of manufacturing biosensor devices for sequencing DNA or RNA and analyzing biomolecules.

Various aspects of the present disclosure provide methods, apparatus and systems for single-molecule biosensors having nanowire or nanoribbon bridges between electrodes for sequencing and information storage and reading. In various embodiments, the present disclosure provides nanofabrication of biomolecular sensing devices beginning with parallel arrangements of transferable nanowires or nanoribbons, and provides in general methods of manufacturing biosensor devices for sequencing DNA or RNA and analyzing biomolecules.

A structured substrate includes a substrate body having an active side. The substrate body includes reaction cavities that open along the active side and interstitial regions that separate the reaction cavities. The structured substrate includes an ensemble amplifier positioned within each of the reaction cavities. The ensemble amplifier includes a plurality of nanostructures configured to at least one of amplify electromagnetic energy that propagates into the corresponding reaction cavity or amplify electromagnetic energy that is generated within the corresponding reaction cavity.

A structured substrate includes a substrate body having an active side. The substrate body includes reaction cavities that open along the active side and interstitial regions that separate the reaction cavities. The structured substrate includes an ensemble amplifier positioned within each of the reaction cavities. The ensemble amplifier includes a plurality of nanostructures configured to at least one of amplify electromagnetic energy that propagates into the corresponding reaction cavity or amplify electromagnetic energy that is generated within the corresponding reaction cavity.

Disclosed are methods for generating physical maps from feature density profiles of a nucleic acid using a constriction device, and associated methods of analyzing said genomic profiles. In addition, disclosed are devices and methods for analyzing secondary, tertiary and quaternary structures on nucleic acids in spatial and temporal context of the 3-D organization of the genome in a constriction or sensor device.

Disclosed are methods for generating physical maps from feature density profiles of a nucleic acid using a constriction device, and associated methods of analyzing said genomic profiles. In addition, disclosed are devices and methods for analyzing secondary, tertiary and quaternary structures on nucleic acids in spatial and temporal context of the 3-D organization of the genome in a constriction or sensor device.

The present invention relates to methods for creating conductive nanojunctions using metalized or conductive polymer joined to a DNA nanowire in a nanodevice for chemosensing and biosensing.

The present invention relates to methods for creating conductive nanojunctions using metalized or conductive polymer joined to a DNA nanowire in a nanodevice for chemosensing and biosensing.