The disclosure relates to a method for operating a quantitative polymerase chain reaction (qPCR) method, having the following steps: cyclically carrying out qPCR cycles; measuring the fluorescence in each qPCR cycle in order to obtain a qPCR curve of intensity values; determining the reaction efficiency (η) for each cycle; correcting the intensity value of each cycle on the basis of the reaction efficiency (η) determined for the cycle in question in order to obtain a corrected qPCR curve; and operating the qPCR method on the basis of the corrected qPCR curve.

The disclosure relates to a method for operating a quantitative polymerase chain reaction (qPCR) method, having the following steps: cyclically carrying out qPCR cycles; measuring the fluorescence in each qPCR cycle in order to obtain a qPCR curve of intensity values; determining the reaction efficiency (η) for each cycle; correcting the intensity value of each cycle on the basis of the reaction efficiency (η) determined for the cycle in question in order to obtain a corrected qPCR curve; and operating the qPCR method on the basis of the corrected qPCR curve.

The disclosed technology relates to systems and methods for nucleic acid sequencing utilizing a single light source and a single detector. The disclosed technology may use the light source to stimulate a fluorescence emission from a polynucleotide and identify a nucleobase in the polynucleotide based on the intensity of the fluorescence emission received by the detector. The disclosed technology may utilize four types of nucleotide analogs which emit light at four distinguishable levels when excited by the light source. In various embodiments, the four types of nucleotide analogs may be coupled to different fluorophores or the same fluorophore with different probabilities or copy numbers.

The disclosed technology relates to systems and methods for nucleic acid sequencing utilizing a single light source and a single detector. The disclosed technology may use the light source to stimulate a fluorescence emission from a polynucleotide and identify a nucleobase in the polynucleotide based on the intensity of the fluorescence emission received by the detector. The disclosed technology may utilize four types of nucleotide analogs which emit light at four distinguishable levels when excited by the light source. In various embodiments, the four types of nucleotide analogs may be coupled to different fluorophores or the same fluorophore with different probabilities or copy numbers.

The present invention concerns processes for barcoding nucleic acids from single cells and processes for genotyping ingle cells having a phenotype of interest.

The present invention concerns processes for barcoding nucleic acids from single cells and processes for genotyping ingle cells having a phenotype of interest.

Nucleic acid sequencing methods and systems, the systems including nanochannel chip including: a nanochannel formed in an upper surface of the nanochannel chip and; a roof covering the nanochannel and comprising nanopores and a field enhancement structure; and a barrier disposed in the nanochannel. The method including: introducing a buffer solution including long-chain nucleic acids to the nanochannel chip; applying a voltage potential across the nanochannel chip to drive the nucleic acids through the nanochannel, towards the barrier, and to translocate the nucleic acids through nanopores adjacent to the barrier, such that bases of each of the nucleic acids pass through the field enhancement structure one base at a time and emerge onto an upper surface of the roof; detecting the Raman spectra of the bases of the nucleic acids as each base passes through the electromagnetic-field enhancement structure; and sequencing the nucleic acids based on the detected Raman spectra.

An objective lens is used for DNA sequencing. An example system includes a flow cell, the objective lens, and a camera. Light from the flow cell is imaged by the camera through the objective lens. The objective lens can provide a long working distance; a flat field curvature; a high numerical aperture; and/or a wide field of view.

An objective lens is used for DNA sequencing. An example system includes a flow cell, the objective lens, and a camera. Light from the flow cell is imaged by the camera through the objective lens. The objective lens can provide a long working distance; a flat field curvature; a high numerical aperture; and/or a wide field of view.

The present invention concerns processes for barcoding nucleic acids from single cells and processes for genotyping single cells having a phenotype of interest.