A novel method of growing fungi is disclosed which uses an engineered artificial media and produces high density filamentous fungi biomats that can be harvested with a minimum of processing and from which fungal products such as antibiotics, proteins, and lipids can be isolated, the method resulting in lowered fungus cultivation costs for energy usage, oxygenation, water usage and waste stream production.

Disclosed is a filamentous fungus and use thereof in gas metabolism, which belongs to the technical field of microorganisms. The filamentous fungus is an Aspergillus cejpii strain S8 deposited in the China General Microbiological Culture Collection Center (CGMCC), No. 3, No. 1, West Beichen Road, Chaoyang District, Beijing on Sep. 20, 2023, with a deposit number of CGMCC NO. 40828.

Disclosed is a filamentous fungus and use thereof in gas metabolism, which belongs to the technical field of microorganisms. The filamentous fungus is an Aspergillus cejpii strain S8 deposited in the China General Microbiological Culture Collection Center (CGMCC), No. 3, No. 1, West Beichen Road, Chaoyang District, Beijing on Sep. 20, 2023, with a deposit number of CGMCC NO. 40828.

A recombinant microbial host cell having improved in vivo conversion of reticuline and derivatives thereof (such as thebaine and/or oripavine) to relevant downstream opioids (such as neopinone, oripavine, northebaine, nororipavine or morphinone) and related compounds (such as heroin, morphine, codeine, thebaine, oripavine, oxycodone, hydrocodone, hydromorphone, oxymorphone, buprenorphine, naltrexone, naloxone or nalbuphine), wherein the microbial (such as fungal) host cell is heterologously expressing at least one functional transporter protein capable of transporting reticuline or a derivative thereof (such as thebaine and/or oripavine) and a heterologously expressed enzyme capable of acting upon reticuline or a derivative thereof. The invention also relates to uses of the microbial host cells and methods of making an opioid compound and/or opioid precursor compound and/or opioid derivative of interest.

The present application provides an endophytic bacterial strain with a high camptothecin yield, which is Alternaria temuissima ZCMUKL-S1, deposited as CCTCC NO: M2021189, or Alternaria citricancri ZCMUKL-S2, deposited as CCTCC NO: M2021190, or Aspergillus versicolor ZCMUKL-S3, deposited as CCTCC NO: M2021191. The endophytic bacterial strain has a high yield of camptothecin, wherein the yield of camptothecin per unit dry mycelium powder of Aspergillus versicolor ZCMUKL-S3 is as high as 116 g/g, which is the highest yield of camptothecin produced by endophytic bacterial strains reported at present, and has potential application value for its industrial production. In addition, the endophytic bacterial strain which can produce high camptothecin provided by the present application has good genetic stability.