Recombinant Aspergillus genetically modified to increase expression of g8846, renamed herein as aconitic acid exporter (aexA), are provided, which in some examples are also genetically inactivated for an endogenous cis-aconitic acid decarboxylase (cadA) gene. Such recombinant Aspergillus produce more aconitic acid as compared to native Aspergillus. Also provided are methods of using such recombinant Aspergillus to increase production of aconitic acid and other organic acids, such as citric acid, itaconic acid, and 3-hydroxypropionic acid (3-HP).

The present invention provides a method for producing an alkaline phosphatase (ALP), the method including a step of: culturing an Aspergillus transformant capable of producing the ALP.

Provided are an Aspergillus oryzae LGYM4 strain; a red ginseng fermentation product including the strain or a fermentation product thereof; a food composition, a cosmetic composition, a quasi-drug composition, and a pharmaceutical composition, each including the red ginseng fermentation product; a method of preparing the red ginseng fermentation product, the method including the step of performing a fermentation by inoculating the strain into a composition including red ginseng; a red ginseng fermentation product prepared according to the preparation method; and a food composition, a cosmetic composition, a quasi-drug composition, and a pharmaceutical composition, each including the prepared red ginseng fermentation product.

The present invention relates to a method for preparing hot pepper paste (gochujang in Korean) by using a novel strain, and hot pepper paste prepared therefrom. More specifically, the hot pepper paste having a consistent quality and improved flavor and taste can be prepared by isolating and selecting Aspergillus oryzae having remarkable carbohydrate and protein degradation enzymatic activities from traditional meju (fermented soybeans) by using wheat flour or polished wheat as a substrate, and using the isolated and selected product for preparing the hot pepper paste.

Recombinant Aspergillus genetically modified to increase expression of g8846, renamed herein as aconitic acid exporter (aexA), are provided, which in some examples are also genetically inactivated for an endogenous cis-aconitic acid decarboxylase (cadA) gene. Such recombinant Aspergillus produce more aconitic acid as compared to native Aspergillus. Also provided are methods of using such recombinant Aspergillus to increase production of aconitic acid and other organic acids, such as citric acid, itaconic acid, and 3-hydroxypropionic acid (3-HP).

The present invention discloses an Aspergillus oryzae JAAS-32 and use thereof in preparation of a larval Hermetia illucens paste protein peptide. The Aspergillus oryzae JAAS-32 was deposited in Guangdong Microbial Culture Collection Center (GDMCC) on Aug. 10, 2023 with an accession number of GDMCC No: 63725, classified and named Aspergillus oryzae. A larval Hermetia illucens paste is subjected to enzymatic fermentation by the Aspergillus oryzae JAAS-32 provided by the present invention for 20-40 hours. The content of the protein peptide (<10 kDa) is 1.53 times that of an untreated group; the content of free amino acids is 1.41 times that of the untreated group; the total antioxidant activity is 1.35 times that of the untreated group. Diameters of inhibition zones against Staphylococcus aureus, Escherichia coli, and Vibrio alginolyticus are 1.62, 1.17, and 1.45 times those of the untreated group, respectively. The strain is cultured readily, fast in growth, and well-adapted. A low-cost, easy-to-operate, and efficient method for enhancing antibacterial performance of the larval Hermetia illucens paste can be established on the basis of the strain.

The present invention discloses an Aspergillus oryzae JAAS-32 and use thereof in preparation of a larval Hermetia illucens paste protein peptide. The Aspergillus oryzae JAAS-32 was deposited in Guangdong Microbial Culture Collection Center (GDMCC) on Aug. 10, 2023 with an accession number of GDMCC No: 63725, classified and named Aspergillus oryzae. A larval Hermetia illucens paste is subjected to enzymatic fermentation by the Aspergillus oryzae JAAS-32 provided by the present invention for 20-40 hours. The content of the protein peptide (<10 kDa) is 1.53 times that of an untreated group; the content of free amino acids is 1.41 times that of the untreated group; the total antioxidant activity is 1.35 times that of the untreated group. Diameters of inhibition zones against Staphylococcus aureus, Escherichia coli, and Vibrio alginolyticus are 1.62, 1.17, and 1.45 times those of the untreated group, respectively. The strain is cultured readily, fast in growth, and well-adapted. A low-cost, easy-to-operate, and efficient method for enhancing antibacterial performance of the larval Hermetia illucens paste can be established on the basis of the strain.