Pesticidal protein exhibiting toxic activity against Lepidopteran and Hemipteran pest species are disclosed, and include, but are not limited to, TIC2199. DNA constructs are provided which contain a recombinant nucleic acid sequence encoding the disclosed pesticidal protein. Transgenic plants, plant cells, seed, and plant parts resistant to Lepidopteran and Hemipteran infestation are provided which contain recombinant nucleic acid sequences encoding the pesticidal proteins of the present invention. Methods for detecting the presence of the recombinant nucleic acid sequences or the protein of the present invention in a biological sample, and methods of controlling Lepidopteran and Hemipteran species pests using TIC2199 pesticidal protein are also provided.

The present invention provides a method for producing a fermentation product by culturing recombinant exosporium-producing Bacillus cells that express a fusion protein of interest on the exosporium in a medium containing sources of carbon and nitrogen in a total concentration of at least 20 g/L, resulting in a fermentation broth containing a high titer of the recombinant Bacillus spores.

Compositions are provided that include a new Bacillus thuringiensis strain designated RTI545 for use in benefiting plant growth and controlling plant pests. In particular, the RTI545 strain is useful for controlling plant nematode, insect and fungal pests. The compositions include plant seeds coated with the RTI545 strain. The compositions can be applied alone or in combination with other microbial, biological, or chemical insecticides, fungicides, nematicides, bacteriocides, herbicides, plant extracts, plant growth regulators, or fertilizers. In one example, enhanced growth and insect control are provided by delivering at the time of planting a combination of a chemical insecticide such as bifenthrin and a liquid fertilizer to plants or seeds treated with RTI545.

Strain of Bacillus thuringiensis novonest4 and applications thereof. The Bacillus thuringiensis is the Bacillus thuringiensis novonest4, with an Accession No. CCTCC NO: M2018443. The strain is firstly reported to have both the effect of preventing and controlling lepidoptera pests after foilar application and the effects of degrading carbendazim, promoting crop growth and increasing crop yield after soil application, and has wide application prospects in agricultural production.

Water-responsive actuators and methods for creating water responsive actuators are disclosed. In some embodiments, the disclosed subject matter includes a first layer, for example a plastic tape, and a second layer, for example bacterial spores and cured adhesive. The second layer can be created in a pattern. The pattern can include joints, which can contract when exposed to dry air and can thereby bend the actuator, and can expand when exposed to humid air and thereby return the actuator its original position.

Water-responsive actuators and methods for creating water responsive actuators are disclosed. In some embodiments, the disclosed subject matter includes a first layer, for example a plastic tape, and a second layer, for example bacterial spores and cured adhesive. The second layer can be created in a pattern. The pattern can include joints, which can contract when exposed to dry air and can thereby bend the actuator, and can expand when exposed to humid air and thereby return the actuator its original position.

Provided are biologically pure bacterial isolates characterized by a genome structure at least 90% similar to a genome structure of a bacterial species selected from the group consisting of: Streptomyces sp. E128 having an NRRL Accession No. B-67462, Bacillus amyloliquefaciens A190 having an NRRL Accession No. B-67464, Bacillus subtilis P243 having an NRRL Accession No. B-67459, Bacillus thuringiensis M979 having an NRRL Accession No. B-67457, Massilia aurea P63 having an NRRL Accession No. B-67461, Rhodococcus sp. G706, Stenotrophomonas maltophilia E132 having an NRRL Accession No. B-67460, Streptomyces aurantiacus A918, Streptomyces badius 0180, Streptomyces mirabilis B670 having an NRRL Accession No. B67463, Streptomyces scopuliridis F427 having an NRRL Accession No. B-67458, and Streptomyces sp. L219. Also provided are whole cell broth or lysates thereof, and polynucleotide, polypeptides and constructs expressing same, compositions-of-matter comprising same and methods using same for killing or inhibiting the development of insects.

Provided are biologically pure bacterial isolates characterized by a genome structure at least 90% similar to a genome structure of a bacterial species selected from the group consisting of: Streptomyces sp. E128 having an NRRL Accession No. B-67462, Bacillus amyloliquefaciens A190 having an NRRL Accession No. B-67464, Bacillus subtilis P243 having an NRRL Accession No. B-67459, Bacillus thuringiensis M979 having an NRRL Accession No. B-67457, Massilia aurea P63 having an NRRL Accession No. B-67461, Rhodococcus sp. G706, Stenotrophomonas maltophilia E132 having an NRRL Accession No. B-67460, Streptomyces aurantiacus A918, Streptomyces badius O180, Streptomyces mirabilis B670 having an NRRL Accession No. B67463, Streptomyces scopuliridis F427 having an NRRL Accession No. B-67458, and Streptomyces sp. L219. Also provided are whole cell broth or lysates thereof, and polynucleotide, polypeptides and constructs expressing same, compositions-of-matter comprising same and methods using same for killing or inhibiting the development of insects.

Described herein are an immobilized enzyme, and a preparation method therefor and a use thereof. The immobilized enzyme includes activated PEI and an enzyme covalently bonded to the activated PEI, where the enzyme is selected from any one of a transaminase, a ketoreductase, a monooxygenase, an ammonia lyase, an ene-reductase, an imine reductase, an amino acid dehydrogenase and a nitrilase.

The present invention provides a consortium of resistant microorganisms for biodegrading carbohydrates in the organic fraction of any source of solid waste. The genetic, metabolic and morphological structure of these resistant microorganisms works efficiently by biodegrading and mineralising solid biodegradable waste from municipal or harvest solid waste, reducing the production of gases and leachates. This compound enriches and increases the concentration of beneficial microorganisms, generating high-quality biological fertilisers suitable for use in agricultural production, in land recovery and conservation, under the parameters established in sustainable organic farming, which seeks to conserve, recover and use nature or the environment without generating the least negative impact.