The present invention relates to a method for recovering a protein of interest from a bacterial fermentation broth, wherein the bacterial cells in the fermentation broth exhibit a high degree of lysis, comprising the steps of adding to the fermentation broth comprising the protein of interest at least one flocculant in an amount of 0.5 to 50 g/l of the fermentation broth; separating the protein of interest from at least the bacterial cells by filtration, preferably by dead-end filtration or microfiltration; and thereby obtaining the protein of interest in the filtrate; wherein the bacterial cells are Bacillus cells. Further, the invention relates to the use of a flocculant for recovering a protein of interest from a bacterial fermentation broth comprising a high degree of lysed cells.

A method of using lactase for generating galactooligosaccharide as well as the preparation and an application of the lactase are provided. Lactase (BglD305 derived from Bacillus circulans B2301 and BglD derived from Bacillus circulans ATCC 31382) molecules from two sources are taken as the basis for molecular evolution, so as to obtain new lactase enzyme molecules with high galactooligosaccharide synthesis efficiency and good expression performance. The high-producing strain lactase is further constructed, the lactase can be efficiently synthesized during the submerged fermentation, and the enzyme molecule is secreted into the culture medium, the high-activity enzyme preparation is directly prepared from the fermentation supernatant, and the lactase expression level can achieve 2208 U/mL. As the result, the fermentation manufacturing cost of lactase is reduced, the fermentation manufacturing process is simplified, and the quality of the lactase preparation is improved.

A method of using lactase for generating galactooligosaccharide as well as the preparation and an application of the lactase are provided. Lactase (BglD305 derived from Bacillus circulans B2301 and BglD derived from Bacillus circulans ATCC 31382) molecules from two sources are taken as the basis for molecular evolution, so as to obtain new lactase enzyme molecules with high galactooligosaccharide synthesis efficiency and good expression performance. The high-producing strain lactase is further constructed, the lactase can be efficiently synthesized during the submerged fermentation, and the enzyme molecule is secreted into the culture medium, the high-activity enzyme preparation is directly prepared from the fermentation supernatant, and the lactase expression level can achieve 2208 U/mL. As the result, the fermentation manufacturing cost of lactase is reduced, the fermentation manufacturing process is simplified, and the quality of the lactase preparation is improved.

A method for controlling self-propelled particles includes providing the particles to a liquid crystalline medium having predesigned local ordering. The method may control at least one of: a local concentration, trajectory, and net flow of self-propelled particles.

A method of producing Bacillus spores comprising a step of culturing the Bacillus bacterium using a liquid medium having a C/N ratio (weight ratio of carbon content to nitrogen content) of greater than 4 and less than 9.5.

The present invention relates to compositions and methods for increasing the health of aquatic animals comprising one or more selected bacterial strains.

Compositions and methods for increasing the health of aquatic animals, including fish and shrimp, through the use of Bacillus strains capable of controlling pathogenic microorganisms in the gut of aquatic animals, reducing inflammation in the gut of aquatic animals, and increasing the surface area and length of intestinal villi in the gut of aquatic animals.

The present technology generally relates to a microorganism displaying antigenic proteins of the SARS-CoV2 coronavirus on its surface, to methods of preparing same, to composition comprising such microorganism and to methods for treatment of SARS-CoV2 related infections in subjects.

A method for controlling self-propelled particles includes providing the particles to a liquid crystalline medium having predesigned local ordering. The method may control at least one of: a local concentration, trajectory, and net flow of self-propelled particles.

A method of producing Bacillus spores comprising a step of culturing the Bacillus bacterium using a liquid medium having a C/N ratio (weight ratio of carbon content to nitrogen content) of greater than 4 and less than 9.5.