The present disclosure is in the technical field of synthetic biology and metabolic engineering. The disclosure provides engineered viable bacteria. In particular, the disclosure provides viable bacteria with reduced cell wall biosynthesis additionally modified for production of glycosylated product. The disclosure further provides methods of generating viable bacteria and uses thereof. Furthermore, the disclosure in the technical field of fermentation of metabolically engineered microorganisms producing glycosylated product.

The present disclosure provides an attenuated Nocardia seriolae and its construction method and use thereof, which belongs to the technical field of genetic engineering. In the present disclosure, part or all of the glutamate endopeptidase homologue (GluNS) gene sequence of wild-type Nocardia seriolae is knocked out by means of genetic engineering to construct an attenuated strain with the characterized of good protection to the host. The attenuated strain may not only effectively reduce the pathogenicity of the bacteria, but also retain good immunogenicity. At the same time, the attenuated Nocardia seriolae constructed in the present disclosure may also have the characteristics of high genetic stability and may be used as a vaccine candidate strain for the preparation of a vaccine or other biological products for preventing and treating Nocardiosis.

In an embodiment, the present disclosure pertains to an oleaginous bacterium. In some embodiments, the oleaginous bacterium includes lipids and at least one exogenous and inducible gene. In some embodiments, the exogenous and inducible gene encodes at least one protein capable of inducing lysis in the oleaginous bacterium to release the lipids. In an addition embodiment, the present disclosure pertains to a method of releasing lipids into an environment. In general, the method includes one or more of the following steps of: (1) introducing at least one oleaginous bacterium of the present disclosure to the environment; and (2) inducing expression of at least one exogenous gene in the oleaginous bacterium to thereby induce the expression of at least one protein. In some embodiments, the protein facilitates the lysis of the oleaginous bacterium and release of the lipids into the environment.

Provided is a method of diagnosing or treating a disease in a subject using the vesicles derived from bacteria of the genus Rhodococcus. The vesicles are significantly decreased in a clinical sample obtained from a patient with lung cancer, pancreatic cancer, bile duct cancer, breast cancer, bladder cancer, lymphoma, diabetes, stroke, myocardial infarction, asthma, COPD, and dementia, compared with a normal individual, and administration of the vesicles to the patient inhibits the secretion of inflammation mediators caused by pathogenic vesicles such as E. coli-derived vesicles, and inhibits cranial nerve cell damage caused by stress hormones, therefore the vesicles are used in a method of diagnosing lung cancer, pancreatic cancer, bile duct cancer, breast cancer, bladder cancer, lymphoma, diabetes, stroke, myocardial infarction, asthma, COPD, and/or dementia, and a method of alleviating or treating a malignant disease, diabetes, stroke, a cardiovascular disease, an inflammatory pulmonary disease, and/or a cranial nerve disease.

The present invention concerns a genetically modified bacterium and to its industrial application, in particular in the 1,2-dehydrogenation of steroids.

The present disclosure is directed to composition and methods for use in drug screening methods. The identification of microbial strains producing antibiotics effective against multi-drug resistant bacterial pathogens using microfluidics and co-encapsulation of predator and prey strains permits rapid and multiplexed assessment of new antibiotics for emerging bacterial pathogens including those resistant to multiple existing antibiotics.

The present disclosure provides engineered bacteria for producing vanillin.

A genetically-modified bacterium, for example of the class Actinobacteria, and the use of such a bacterium in the bioconversion of a steroidal substrate into a steroidal product of interest. A method of converting a steroidal substrate into a steroidal product of interest, wherein the method comprises: inoculating culture medium with genetically-modified bacteria according to any of Claims 1 to 28 and growing the bacterial culture until a target OD.sub.600 is reached; adding a steroidal substrate to the bacterial culture when the target OD.sub.600 is reached; culturing the bacterial culture so that the steroidal substrate is converted to the steroidal product of interest; and extracting and/or purifying the steroidal product of interest from the bacterial culture.

A bacterial strain of Rhodococcus biphenylivorans named Palladio 22 and registered at the BCCM-LMG Bacteria Collection under registration number LMG P-29520. A method is provided for the production of acrylamide following hydration of acrylonitrile using a biomass of the bacterial strain.

This invention teaches systems and methods for identifying, targeting and destroying cancer cells. As cells progress from a normal to a cancerous state their accelerated metabolic rates and adapted pathways generate a higher heat signature that serves as a targeting beacon for a specialized cell killing vector. Suitable vectors include modified or adapted viruses, modified or adapted intracellular bacteria and/or engineered liposomes. Especially preferred is the bacterial vector because of its ease of production. The bacterial vector is selectively targeted to recognize cells whose temperature is slightly elevated and ambient pH suppressed due to cancer related alterations to metabolism. An additional targeting feature, such as recognition of the MCT4 transmembrane protein exaggeratively expressed on the cancer cell outer membrane, may provide additional targeting specificity. Embodiments featuring facultative extracellular and intracellular growth capable bacteria have the preferred feature that culture conditions for producing the vector can be optimized solely for the one organism and need not be compromised to support or optimize host cell maintenance.