Soil plant productivity is improved by manipulation of soil microflora. The soil microflora is manipulated by addition of a bacterium belonging to Rhizobium (Rhizobiales) to the soil.

The disclosure relates to isolated microorganisms—including novel strains of the microorganisms—microbial consortia, and agricultural compositions comprising the same. Furthermore, the disclosure teaches methods of utilizing the described microorganisms, microbial consortia, and agricultural compositions comprising the same, in methods for imparting beneficial properties to target plant species. In particular aspects, the disclosure provides methods of increasing desirable plant traits in agronomically important crop species.

The present application relates to plant growth promoting microbes (PGPMs), compositions comprising these PGPMs and methods of using these PGPMs and/or compositions for enhancing plant health, plant growth and/or plant yield, and/or for preventing, inhibiting, or treating the development of plant pathogens or the development of phytopathogenic diseases. This application also provides non-naturally occurring plant varieties that are artificially infected with a PGPM descried herein, as well as seed, reproductive tissue, vegetative tissue, regenerative tissues, plant parts, or progeny thereof.

The present invention relates to excision of explant material comprising meristematic tissue from seeds, and storage of such material prior to subsequent use in plant tissue culture and genetic transformation. Methods for tissue preparation, storage, and transformation are disclosed, as is transformable meristem tissue produced by such methods, and apparati for tissue preparation.

The present invention relates to excision of explant material comprising meristematic tissue from cotton seeds. Methods for tissue preparation, storage, transformation, and selection or identification of transformed plants are disclosed, as are transformable meristem tissues and plants produced by such methods, and apparati for tissue preparation.

Provided herein is a method for producing a lyophilized composition of viable cells of Rhizobium bacteria having high viable cell viability, and a lyophilized composition of viable cells of Rhizobium bacteria having desirable long-storage survivability, among others. The lyophilized composition of viable cells of Rhizobium bacteria having high viable cell viability and desirable long-storage survivability can be obtained when a composition containing viable cells of Rhizobium bacteria and more than 10 mass % moisture is lyophilized to bring the moisture content to 10 mass %, and the drying is ended before the moisture content becomes less than 5 mass % so that the composition after the lyophilization contains the viable bacteria and 5 to 10 mass % of moisture.

Pesticidal proteins exhibiting toxic activity against Lepidopteran pest species are disclosed, and include, but are not limited to, TIC13085 and TIC13087. DNA constructs are provided which contain a recombinant nucleic acid sequence encoding one or more of the disclosed pesticidal proteins. Transgenic plants, plant cells, seed, and plant parts resistant to Lepidopteran infestation are provided which contain recombinant nucleic acid sequences encoding the pesticidal proteins of the present invention. Methods for detecting the presence of the recombinant nucleic acid sequences or the proteins of the present invention in a biological sample, and methods of controlling Lepidopteran species pests using any of the TIC13085 and TIC13087 pesticidal proteins are also provided.

The present invention relates to excision of explant material comprising meristematic tissue from cotton seeds. Methods for tissue preparation, storage, transformation, and selection or identification of transformed plants are disclosed, as are transformable meristem tissues and plants produced by such methods, and apparati for tissue preparation.

Provided is a type of Rhizobium with the classified nomenclature of BradyRhizobium sp. KTMS 0001 or BradyRhizobium sp. KTMS 0002, and the deposit number of CCTCC No. M2017580 or CCTCC No. M2017581. Also provided are a bacterial preparation containing the Rhizobium, a method for restoring rare-earth tailings soil and a use of the Rhizobium.

Methods, compositions, systems and kits relating to processing of cassava bagasse into bacterial feedstock, such as bacterial feedstock suitable for nanocellulose production, are disclosed. Cassava bagasse may be contacted with an acid catalyst or an enzymatic catalyst to produce a hydrolysate, which can be used to form a pre-fermentation medium. Incubation of the pre-fermentation medium with a first population of microorganisms yields a supernatant enriched in reducing sugars, which may be used to form a culture medium which can be used to support growth of a second population of microorganisms to form the nanocellulose.