The present disclosure discloses a visualized screening method for an Aspergillus recombinant strain with multigene editing and belongs to the technical field of gene engineering. CRISPR-Cas9 is used in the disclosure to cleave spore color change-related genes and a target gene in Aspergillus at the same time, such that editing of the target gene is visualized and an Aspergillus niger strain with multigene editing can be rapidly and efficiently screened out through spore phenotypes. Through different combinations of visualized genes and non-phenotypic change genes, rapid screening of the strain with multigene editing and simultaneous screening of multiple visualized genes are realized, and use of resistance genes in industrial strains is reduced.

A method for determining a level of one or more live microbes in a sample of a Cannabis plant includes (a) introducing a DNase enzyme into a sample including (i) Cannabis plant matrix derived from lysed cells of a Cannabis plant and (ii) live microbial cells including microbial genomic DNA, (b) introducing an anionic detergent into the sample, where the anionic detergent is in an amount effective to inactivate the DNase enzyme in the sample and lyse the live microbial cells in the sample, (c) introducing a known quantity of control DNA into the sample, (d) contacting the sample with a plurality of polynucleotide capture agents, (e) isolating the plurality of polynucleotide capture agents, thereby isolating polynucleotides from the microbial genomic DNA and/or control DNA in the sample, and (f) amplifying at least a portion of polynucleotides from the microbial genomic DNA isolated in step (e).

The present invention relates to recombinant filamentous fungal host cells producing cellulolytic enzyme compositions and methods of producing and using the compositions.

The present application relates to methods for detecting a tandem repeat in a nucleic acid sequence under isothermal conditions using primers.

The present invention relates to recombinant filamentous fungal host cells producing cellulolytic enzyme compositions and methods of producing and using the compositions.

The present disclosure relates to kits, compositions and methods for detecting fungal species, viruses and/or protein variants in a sample, e.g., a biological sample. For example, but not by way of limitation, the present disclosure provides living yeast biosensors that have been genetically engineered to detect fungal species, viruses and/or protein variants in a sample, e.g., a biological sample.

The present invention relates to recombinant filamentous fungal host cells producing cellulolytic enzyme compositions and methods of producing and using the compositions.

An Aspergillus fumigatus for degrading soil microplastics and an application thereof are provided. The Aspergillus fumigatus has a preservation name of Aspergillus fumigatus F and is deposited at the China General Microbiological Culture Collection Center on Sep. 18, 2024 under CGMCC NO. 41513. The Aspergillus fumigatus F may grow in an environment with PE and PET as the only carbon sources, and may be used as a biodegradable microbial inoculum applied to harmless treatment, recycling and environmental restoration of microplastic resources.

A method for determining a level of one or more live microbes in a sample of a Cannabis plant includes (a) introducing a DNase enzyme into a sample including (i) Cannabis plant matrix derived from lysed cells of a Cannabis plant and (ii) live microbial cells including microbial genomic DNA, (b) introducing an anionic detergent into the sample, where the anionic detergent is in an amount effective to inactivate the DNase enzyme in the sample and lyse the live microbial cells in the sample, (c) introducing a known quantity of control DNA into the sample, (d) contacting the sample with a plurality of polynucleotide capture agents, (e) isolating the plurality of polynucleotide capture agents, thereby isolating polynucleotides from the microbial genomic DNA and/or control DNA in the sample, and (f) amplifying at least a portion of polynucleotides from the microbial genomic DNA isolated in step (e).