A method for producing a mutant filamentous fungus. The method comprises modifications of XYR1 and ACE3 expression in the parent filamentous fungus. The modification of XYR1 is substitution, deletion, insertion, or addition of at least one amino acid residue in a region corresponding to positions 810 to 833 of SEQ ID NO: 1 in a polypeptide that consists of the amino acid sequence of SEQ ID NO: 1 or an amino acid sequence having at least 90, identity thereto and functions as a transcriptional activator of cellulase and hemicellulase, and the modification of ACE3 expression is enhanced expression of a partial polypeptide of ACE3.

The invention relates to a strain of fungus having a reduced viscosity, wherein the ID 78713 (GEL3) gene has been invalidated. The invention also relates to the different uses of this strain, as well as to the method of genetic modification.

A process for producing cellulolytic and/or hemicellulolytic enzymes with a strain of microorganism belonging to the family of filamentous fungi. The process includes growing the fungi in an aqueous culture medium, in the presence of at least one carbon-based growth substrate, in a stirred and aerated bioreactor. It also includes the production of enzymes, starting with the aqueous culture medium in the presence of at least one inductive carbon-based substrate and also inducing the production of hydrophobins. Further, at least a portion of the hydrophobins produced in step (b) are reintroduced into the growth step (a).

A biological inoculant having both fertilizing and fungicidal activity is disclosed. More particularly, a bio-inoculant having the said combined effects, comprising Bradyrhizobium japonicum and specific isolates from the Trichoderma genus is disclosed. The bio-inoculant is applied to soybean crops for preventing fungi borne diseases. More particularly, the bio-inoculant of the invention is useful for protecting soybean crops against infection by Fusarium sp., Colletotrichum sp., Cercospora sp., Sclerotinia sp. and Rhizoctonia sp.

A Trichoderma reesei mutant strain has a function of a polypeptide consisting of the amino acid sequence represented by SEQ ID NO: 2 is reduced. A method of producing a protein includes a step of cultivating the Tricho-derma reesei mutant strain, and a method of producing a cellulase includes a step of cultivating the Trichoderma reesei mutant strain.

A mutant strain of a filamentous fungus of the genus Trichoderma having a reduced function of a polypeptide consisting of the amino acid sequence represented by SEQ ID NO: 2; and a method of producing a sugar from a cellulose-containing biomass, the method including: step a of producing a cellulase by cultivating a Trichoderma reesei mutant strain having a reduced function of a polypeptide consisting of the amino acid sequence represented by SEQ ID NO: 2, and step b of saccharifying the biomass by using the cellulase obtained in the step a.

The present invention provides a method for making a consortium of fungi resistant to traces of carbamates contained in thiodicarb and to traces of synthetic pyrethroids contained in bifenthrin. During the course of this research, carried out over more than five years, the strains were subjected to a change in physical medium, adapting same to tolerate increasing concentrations of thiodicarb and bifenthrin. This compound enriches and increases the concentration of beneficial microorganisms, generating high-quality liquid biological fertilisers suitable for use in agricultural production, in land recovery and conservation, under the parameters established in sustainable organic farming, which seeks to conserve, recover and use nature or the environment without generating the least negative impact.

The purpose of the present invention is to provide a method for producing selenoneine that allows production of selenoneine at higher yields, even if an inorganic selenium compound is used as a selenium compound. This purpose can be achieved by a method for producing selenoneine, comprising the step of applying histidine and a selenium compound to a transformant to obtain selenoneine, wherein the transformant has at least one gene selected from the group consisting of a SatA gene, a CysB gene and a MetR gene, and an EgtA gene inserted therein and can overexpress the inserted genes.

The present disclosure relates to formulations including Trichoderma isolates, as well as methods thereof. In particular embodiments, the formulation can include one or more Trichoderma isolates, in which the formulation can include solid carriers and/or liquid carriers. Such formulations can be useful for treating or protecting plants, as well as counteracting growth of pathogenic organisms in proximity to the plant.

A novel method of growing fungi is disclosed which uses an engineered artificial media and produces high density filamentous fungi biomats that can be harvested with a minimum of processing and from which fungal products such as antibiotics, proteins, and lipids can be isolated, the method resulting in lowered fungus cultivation costs for energy usage, oxygenation, water usage and waste stream production.