A novel method of growing fungi is disclosed which uses an engineered artificial media and produces high density filamentous fungi biomats that can be harvested with a minimum of processing and from which fungal products such as antibiotics, proteins, and lipids can be isolated, the method resulting in lowered fungus cultivation costs for energy usage, oxygenation, water usage and waste stream production.

The present disclosure is generally related to mutant and genetically modified filamentous fungal cells and methods thereof for use in the production of proteins of interest. More particularly, as described herein, the mutant and/or modified fungal cells (strains) of the disclosure are well-suited for use in industrial scale fermentation processes for the enhanced expression/production of proteins of interest in the absence and/or in the presence of an inducing substrate.

A novel method of growing fungi is disclosed which uses an engineered artificial media and produces high density filamentous fungi biomats that can be harvested with a minimum of processing and from which fungal products such as antibiotics, proteins, and lipids can be isolated, the method resulting in lowered fungus cultivation costs for energy usage, oxygenation, water usage and waste stream production.

Provided are methods for producing food products comprising recombinant components, and compositions used in and food products produced by such methods.

Methods are provided for enhancing the health, growth and/or sturdiness of turf grasses by applying a combination of microbes and/or their growth by-products to the turf grass roots and/or to soil. Specifically, in one embodiment, the subject invention utilizes Trichoderma harzianum and a strain of Bacillus amyloliquefaciens. The methods can further be used to improve maintenance and upkeep of natural turf grass fields used in, for example, athletics and recreation, as well as to increase the functionality and safety of these natural turf grass fields.

Disclosed are an antiviral composition, a composition for controlling plant viruses, and a method for controlling plant viruses using the composition. Trichodermin or Trichoderminol which is a tricothecene-based compound and is isolated from Trichoderma albolutescenes strain has antiviral activities against various plant viruses and is thus suitable for controlling plant viruses. This description can facilitate mass production of an active component derived from natural products, provide environment-friendly antifungal agents, which do not harm plants, using safe materials, and be variously utilized in agricultural fields, for example, such as production of high-value crops.

The present invention relates to the field of genetic engineering, particularly to a recombinant expression vector for rapidly screening the high expression strains and a method for rapidly screening high expression strains. In the invention, an exogenous red fluorescent protein and Aspergillus fumigatus cell surface protein localization signal are fused and expressed, and the fusion gene (DsRed-AfMP1) is integrated into the genome of Trichoderma reesei, so as to construct a strain displaying red fluorescent protein on the surface of Trichoderma reesei. By sorting Trichoderma reesei strains with red fluorescent protein on the surface by flow cytometry, genes beneficial to the improvement of cellulase activity can be quickly isolated.

A mutant strain of Trichoderma reesei has a mutation that eliminates or reduces a function of a polypeptide consisting of the amino acid sequence represented by SEQ ID NO: 8, in which the mutation may be a mutation in which an aspartic acid residue at the 1,790 residue from the N-terminal side in the polypeptide consisting of the amino acid sequence represented by SEQ ID NO: 8 is changed to a residue of an amino acid other than aspartic acid.

The invention relates to a strain of Trichoderma reesei BLCY-007 and its application in the production of xylooligosaccharides.

A mutant strain of Trichoderma reesei has a mutation that eliminates or reduces a function of a polypeptide consisting of the amino acid sequence represented by SEQ ID NO: 2. A method produces a protein, the method including a step of cultivating the mutant strain.