The present disclosure provides a method for genetically engineering Yarrowia lipolytica host cell for producing glycolic acid from organic wastes. A subject genetically engineered Y. lipolytica cell comprises the disrupted native genes encoding malate synthase, heterologous enzyme of glyoxylate reductase targeted in the different cellular compartments including mitochondria, peroxisome and cytosol, and a mutant NADP.sup.+-dependent malate dehydrogenase. The pathway with a theoretical yield as high as that 1 g of acetic acid can be converted to 1.27 g of glycolic acid without carbon loss was engineered for glycolic acid production. The methods particularly include process for production of volatile fatty acids (VFAs) mainly comprised of acetic acid from organic waste, and then use of resultant VFAs for biosynthesis of glycolic acid by recombinant Y. lipolytica.

Recombinant microorganisms, plants, and plant cells are disclosed that have been engineered to have reduced levels or activity of one or more alcohol dehydrogenases or aldehyde reductases thereby increasing the production of benzylisoquinoline alkaloids and/or benzylisoquinoline alkaloid precursors.

The present invention relates to autotrophic microorganisms with altered photorespiration and improved CO.sub.2 fixation as well as a method of producing said autotrophic microorganisms. Particularly, the autotrophic microorganisms show an improved growth rate, productivity and energy conversion efficiency.

Recombinant microorganisms, plants, and plant cells are disclosed that have been engineered to have reduced levels or activity of one or more alcohol dehydrogenases or aldehyde reductases thereby increasing the production of benzylisoquinoline alkaloids and/or benzylisoquinoline alkaloid precursors.

The present invention relates to autotrophic microorganisms with altered photorespiration and improved CO.sub.2 fixation as well as a method of producing said autotrophic microorganisms. Particularly, the autotrophic microorganisms show an improved growth rate, productivity and energy conversion efficiency.

Recombinant microorganisms, plants, and plant cells are disclosed that have been engineered to have reduced levels or activity of one or more alcohol dehydrogenases or aldehyde reductases thereby increasing the production of benzylisoquinoline alkaloids and/or benzylisoquinoline alkaloid precursors.

Recombinant microorganisms, plants, and plant cells are disclosed that have been engineered to have reduced levels or activity of one or more alcohol dehydrogenases or aldehyde reductases thereby increasing the production of benzylisoquinoline alkaloids and/or benzylisoquinoline alkaloid precursors.

Some aspects provide engineered microbes for glycolate production. Methods for microbe engineering and culturing are also provided herein. Such engineered microbes exhibit greatly enhanced capabilities for glycolate production.

Some aspects provide engineered microbes for glycolate production. Methods for microbe engineering and culturing are also provided herein. Such engineered microbes exhibit greatly enhanced capabilities for glycolate production.

Quantum dots (QDs) and nanoplatelets (NPLs) are two types of nanoparticles used as scaffolds for enzymes operating in enzymatic cascades. Combinations of QDs and NPLs were surprisingly found to operate synergistically to create a greater enhancement than either alone when operating as scaffolds for enzymatic cascade reactions. A process involves providing an enzymatic cascade including a cluster of nanoparticles including both QDs and NPLs and having a plurality of enzymes bound thereto, the enzymes configured as an enzymatic cascade, such that the product of a first enzyme is a substrate of a second enzyme; contacting the cascade cluster with a substrate of the first enzyme; and allowing a reaction to proceed so that each of the plurality of enzymes acts in succession to produce an end product. The enzymes are bound to the nanoparticles via metal affinity coordination between histidine tags on the enzymes and zinc-containing surfaces of the nanoparticles.