The present invention relates to KatG enzymes and enzyme compositions and their uses in enzymatic degradation of plastics.

Animal feeds are preserved using an enzymatic antioxidant. Polypeptides having catalase activity and/or superoxide dismutase activity preserve animal feed or animal feed additives or prevent the degradation of the proteins, vitamins fats and lipids contained in animal feed components.

Animal feeds comprising probiotics are preserved using an enzymatic antioxidant. Polypeptides having catalase activity and/or superoxide dismutase activity preserve and promote probiotic growth and activity in animal.

Novel superoxide dismutases of fungal origin are active, gastric stable and thermal stable, and effective for use in animal feed additives. The use of superoxide dismutase in animal feed improve animal growth, animal health and intestinal health of animals.

The present invention provides for a method for producing hydrogen peroxide comprising: (a) contacting a methanol with a methanol oxidase bound with a flavin adenine dinucleotide (FAD) cofactor, such that the methanol is oxidized into a formaldehyde and the FAD cofactor is reduced into FADH.sub.2; (b) contacting the formaldehyde with the methanol oxidase or a formate oxidase bound with a FAD cofactor, such that the formaldehyde is oxidized into a formate and the FAD is reduced into FADH.sub.2; and (c) contacting oxygen with one or more of the FADH.sub.2 to produce hydrogen peroxide and oxidize FADH.sub.2 into FAD. The present invention also provides for a fusion protein comprising any two or all of methanol oxidase, formate oxidase, and formaldehyde dismutase.

An enzyme-containing composition having a ratio of a protease activity to a peroxidase activity of 10 or less. A method for producing milk including sterilizing raw milk, and filling a container with the sterilized raw milk and then sealing the container, where the enzyme-containing composition is added to the sterilized raw milk during a period after the sterilizing and before a completion of the filling.

Disclosed are a self-assembly enzyme system supplying -ketoglutarate (-KG) and application thereof in catalytic synthesis of 4-hydroxyisoleucine. In the present disclosure, glutamate oxidase catalyzes glutamate to generate -KG, and catalase-peroxidase decomposes a byproduct H.sub.2O.sub.2. An interaction between RIAD and RIDD and a covalently linked combined state can mediate higher-order structures of various self-assembly enzymes. The LGOX/KatG self-assembly system is constructed through the affinity of short peptides in vitro to eliminate H.sub.2O.sub.2 in situ, thereby eliminating the inhibitory effect of H.sub.2O.sub.2 on Fe(II)/-KG DOs, and facilitating efficient and high-yield production of 4-HIL in a one-pot cascade reaction with IDO, with a highest yield up to 95% at a substrate concentration of 100 mM.