Provided are non-naturally occurring nucleic acids comprising a sequence encoding an enzyme or regulatory protein in tryptamine metabolism. Also provided are a recombinant microorganisms expressing the enzyme or regulatory protein. Methods of expressing the enzyme or regulatory protein are additionally provided.

The present invention is directed to all aspects of novel methyl transferase enzymes that methylate backbone amides of (poly)peptides. The present invention also relates to nucleic acids encoding these enzymes as well as corresponding vectors and host cells comprising these. Moreover, the present invention encompasses the use of said enzymes for modifying (poly)peptides as well as corresponding methods. Also, the present invention pertains to further novel enzymes for modifying (poly)peptides derived from the omphalotin gene cluster of O. olearius and the homologous gene clusters from D. bispora, L. edodes and F. mediterranea as well as related aspects.

Disclosed are methods for converting an alkaloid substrate compound into a N-alkylated alkaloid product compound in the presence of an alkyl donor compound and catalytic quantities of an N-alkyltransferase enzyme capable of converting the alkaloid substrate into an alkylated product. The N-alkyltransferase is obtainable from Ephedra sinica.

Provided herein are microorganisms for producing substituted tryptamines and cell cultures thereof, the microorganisms comprising at least one exogenous nucleic acid molecule that encodes at least one substituted tryptamine biosynthetic pathway enzyme.

Disclosed herein are enzymatic and modified host cell methods for prepared modified indole alkaloids. Also disclosed herein are modified indole alkaloids and their therapeutic use for treating diseases and disorders.

The present invention is directed to all aspects of novel methyl transferase enzymes that methylate backbone amides of (poly)peptides. The present invention also relates to nucleic acids encoding these enzymes as well as corresponding vectors and host cells comprising these. Moreover, the present invention encompasses the use of said enzymes for modifying (poly)peptides as well as corresponding methods. Also, the present invention pertains to further novel enzymes for modifying (poly)peptides derived from the omphalotin gene cluster of O. olearius and the homologous gene clusters from D. bispora, L. edodes and F. mediterranea as well as related aspects.

Ways of making and using a recombinant organism configured to produce one or more substituted tryptamines, such as psilocin and psilocybin, are provided. The recombinant organism can include a eukaryotic microorganism expressing a recombinant construct. A substituted tryptamine can be produced by a process that includes growing the recombinant organism configured to produce the substituted tryptamine in a growth medium and separating the substituted tryptamine from the recombinant organism and the growth medium. A biosynthetic system for producing a substituted tryptamine is provided that includes a bioreactor, the recombinant organism configured to produce the substituted tryptamine, and a growth medium for the recombinant organism.