The present invention relates to a method for preparing a plasmid containing DNA that encodes a Type I polyketide synthase (PKS), the method including a step of introducing a DNA construct containing tandem repeats of DNA that encodes a PKS into a Bacillus subtilis competent cell.

The present invention provides for a polyketide synthase (PKS) capable of synthesizing a carboxylic acid, said PKS comprising a synthetic or hybrid module. The present invention also provides for a host cell comprising the PKS and when cultured produces the carboxylic acid. In some embodiments, the carboxylic acid is adipic acid.

The present invention relates to a method for producing polyphenol compounds, i.e. phloroglucinol or one of its derivatives, with a polyketide synthase of type III (PKSIII) from a brown marine alga. The invention also relates to recombinant nucleic acids coding for a polyketide synthase of type III (PKSIII) from the brown alga Ectocarpus siliculosus (E. siliculosus), to recombinant vectors comprising these nucleic acids, as well as to host cells comprising these vectors. Finally, the invention relates to a method for preparing of various compounds by means of polyphenol compounds produced according to the aforementioned method.

The present invention provides for a polyketide synthase (PKS) capable of synthesizing a carboxylic acid, said PKS comprising a synthetic or hybrid module. The present invention also provides for a host cell comprising the PKS and when cultured produces the carboxylic acid. In some embodiments, the carboxylic acid is adipic acid.

Described are recombinant Escherichia coli Nissle 1917 (EcN) cells transformed with genes that express proteins or polypeptides involved in omega 3 fatty acid biosynthesis. The recombinant EcN cells produce eicosapentaenoic acid (EPA) and/or docosahexaenoic acids (DHA). Associated compositions, methods and uses of the recombinant EcN cells are also provided.

The disclosure provides methods for increasing saturated, monounsaturated, and poly unsaturated fatty acid production using the PUFA gene cluster plasmid and the gene regulator pfaR.