The present disclosure provides methods for biosynthesis of acetaminophen. The present disclosure provides host cells genetically modified to provide for production of acetaminophen. The present disclosure provides a recombinant host cell that is genetically modified with one or more heterologous nucleic acids comprising nucleotide sequences encoding 4-aminobenzoate hydroxylase (4ABH) and N-hydroxyarylamine O-acetyltransferase (NhoA). The present disclosure provides a recombinant prokaryotic host cell that is genetically modified with one or more heterologous nucleic acids comprising nucleotide sequences encoding 44ABH and NhoA.

The present disclosure provides compositions and methods for the biosynthetic production of acetaminophen, p-aminophenol, and p-aminobenzoic acid and the purification of biologically derived acetaminophen.

The present invention provides a method for conveniently and inexpensively producing an amino-hydroxybenzoic acid-type compound such as 3-amino-4-hydroxybenzoic acid by utilizing Escherichia coli that is a bacterium commonly used for a production process by biosynthesis. Specifically, the present invention provides an Escherichia coli having an ability to produce 3-amino-4-hydroxybenzoic acid, which is modified to reduce an activity of N-hydroxyarylamine O-acetyltransferase (NhoA); a method for producing a 3-amino-4-hydroxybenzoic acid-type compound using such Escherichia coli; and the like.