The present invention relates to genetically modified ascomycetous filamentous fungi, particularly of the species Thermothelomyces heterothallica, capable of producing elevated amounts of nicotinamide riboside.

The invention relates to a genetically modified prokaryotic cell capable of improved iron-sulfur cluster delivery, characterized by a modified gene encoding a mutant Iron Sulfur Cluster Regulator (IscR) and one or more transgenes or upregulated endogenous genes encoding iron-sulfur (Fe—S) cluster polypeptides or proteins that catalyze complex radical-mediated molecular rearrangements, electron transfer, radical or non-redox reactions, sulfur donation or perform regulatory functions. The prokaryotic cells are characterized by enhanced activity of these iron-sulfur (Fe—S) cluster polypeptides, enhancing their respective functional capacity, and facilitating enhanced yields of compounds in free and protein-bound forms, including heme, hemoproteins, tetrapyrroles, B vitamins, amino acids, δ-aminolevulinic acid, biofuels, isoprenoids, pyrroloquinoline quinone, ammonia, indigo, or their precursors, whose biosynthesis depends on their activity. The invention further relates to a method for producing said compounds or their precursors using the genetically modified prokaryotic cell of the invention, and the use of the genetically modified prokaryotic cell.

The present invention relates to microbial host cells that have been engineered for increased tolerance to temperature shifts, for increased performance at temperatures different from the microorganism's optimal temperature and/or for changing at least one of the microorganism's cardinal temperatures by replacing an endogenous NAD.sup.+ biosynthesis gene by a heterologous gene encoding a corresponding enzyme with another temperature profile and/or from a microorganism with a different optimum growth temperature. The invention further relates to processes wherein the engineered microbial host cells are used for producing a fermentation product, and to the use nucleotide sequences encoding NAD.sup.+ biosynthesis gene for changing at least one of a microorganism's cardinal temperatures and/or for improving a microorganism's tolerance to temperature shifts.

The present invention relates to genetically modified ascomycetous filamentous fungi, particularly of the species Thermothelomyces heterothallica, capable of producing elevated amounts of nicotinamide riboside.

The present invention relates to a recombinant microorganism producing quinolinic acid, more particularly, a microorganism producing quinolinic acid and having attenuated activity or eliminated activity of a protein having a sequence of SEQ ID NO: 1 and a method of producing quinolinic acid by using the recombinant microorganism.

An aspect provides plant cells with an engineered QPT gene and methods of using the same. When a QPT gene in a plant cell is genetically engineered according to an aspect, biosynthesis of nicotine is effectively inhibited, and nicotine is reduced by about 97% or more, and since alkaloids other than nicotine (nornicotine, anabasine, anatabine) are also reduced by about 68% or more, plant cells with reduced carcinogen TSNAs (NNK, NNN, NAB, and NAT) may be produced.

The present invention relates to a novel method, expression vectors, and host cells for producing nicotinic acid riboside by regulating the pathways that lead to the production of nicotinic acid riboside.

The present invention relates to a recombinant microorganism producing quinolinic acid, more particularly, a microorganism producing quinolinic acid and having attenuated activity or eliminated activity of a protein having a sequence of SEQ ID NO: 1 and a method of producing quinolinic acid by using the recombinant microorganism.

The invention relates to a method for metabolic engineering of Actinomycetes. The method is based on traditional mutagenesis combined with reporter-guided single cell technologies. The method may be used for various purposes, such as for producing or increasing yields of endogenous target proteins or secondary metabolites, for replacing medium optimization in the production of target proteins or secondary metabolites, and for activating silent target genes or silent biosynthetic gene cluster. Also provided are metabolically engineered Actinomycetes strains obtainable by the method.

The present disclosure provides engineered bacterial cells having one or more genetic modifications that result in increased production of expression products, such as increased production of plasmids with reduced occurrence of plasmid concatemers or multimers. Also disclosed herein are expression systems for product manufacturing in media having reduced antibiotic concentration, as well as methods of making cells for use in the expression systems. The cells and expression systems increase the growth rate and production yields of biomolecules produced within the host bacterial host cells without the use of antibiotics, and can produce the product with little to no endotoxins present.