The invention provides recombinant microorganisms and methods for the production of acetone from gaseous substrates. For example, the recombinant microorganism may be modified to express an exogenous thiolase, an exogenous CoA transferase, and an exogenous decarboxylase.

A fatty acid decarboxylase is disclosed, the fatty acid decarboxylase comprising at least 40% sequence identity to SEQ ID NO:1 or 2, and an amino acid substitution at a position corresponding to G462 of SEQ ID NO:1.

Provided is a genetically engineered microorganism comprising expression of multiple CoA transferases conferring certain advantages, including increased product production and fermentation stability. Also provided is a method for increasing production of a product comprising culturing the genetically engineered microorganism in the presence of a gaseous substrate wherein the gaseous substrate may comprise a C1-carbon source comprising one or more of CO, CO.sub.2, and H.sub.2.

A fatty acid decarboxylase is disclosed, the fatty acid decarboxylase comprising at least 40% sequence identity to SEQ ID NO:1 or 2, and an amino acid substitution at a position corresponding to G462 of SEQ ID NO:1.

The present invention relates to thermophilic cells and methods for the microbial production of volatile compounds, including acetone, butanone and isopropanol. Also provided are nucleic acid constructs, vectors and host cells useful in such methods.

The present invention provides a method for increasing production of a metabolite by a non-naturally occurring methylotroph, comprising growing the non-naturally occurring methylotroph in a medium comprising methanol. Expression of one or more native genes in the non-naturally occurring methylotroph is changed. Also provided are the non-naturally occurring methylotroph and preparation thereof.

Embodiments of the invention provide a microorganism capable of simultaneous co-fermentation of two or more sugars in a lignocellulosic hydrolysate and having tolerance against microorganism growth inhibitory substances in the lignocellulosic hydrolysate and further having butanol productivity. In addition, embodiments of the invention provide a recombinant microorganism in which a pathway converting butyryl-CoA into butanol or a pathway converting butyrate into butyryl-CoA is promoted, and butanol productivity is increased. Further, a method for producing butanol using the microorganisms is provided.

Disclosed herein are a transformed Synechococcus elongatus strain having improved capability of producing acetone and a method for producing acetone and a method for removing carbon dioxide using the same. In an aspect, the transformed Synechococcus elongatus strain of the present disclosure can produce acetone with high selectivity using carbon dioxide as a carbon source. The present disclosure is economical because the Synechococcus elongatus strain can economically produce high value-added acetone using carbon dioxide existing in the atmosphere as a carbon source without requiring an additional catalytic reaction. Also, the present disclosure is environment-friendly because carbon dioxide in the atmosphere can be removed or reduced using the microorganism.

Disclosed herein are novel methods and compositions of matter to produce 3HB in acetogens by using a(S)-3-hydroxybutyryl-CoA dehydrogenase, Hbd2, responsible for endogenous 3HB production. In conjunction with the heterologous thiolase atoB and CoA transferase ctfAB, hbd2 overexpression improves yields of 3HB on both sugar and syngas (CO/H.sub.2/CO.sub.2), outperforming previously disclosed pathways.