A fermentation process includes contacting a starch hydrolysate with a glucoamylase with agitation. And allowing for settling to form a multi-phase solution. A first phase of the multi-phase solution includes a saccharide component comprising about 30 wt % to about 80 wt % (e.g., 30 wt % to 70 wt %, 30 wt % to 60 wt %) based on a total carbohydrate present. The method further includes draining the first phase to isolate the first phase from a second phase to form a fermentation broth comprising a first portion of the first phase. The method further includes fermenting the fermentation broth until a concentration of glucose in the fermentation broth is 40 g/L or less. The method includes adding a second portion of the first phase to the fermentation broth to maintain a concentration of glucose in a range of from about 1 g/L to about 20 g/L in the fermentation broth.

The present invention relates to processes of recovering oil after liquefaction and/or from thin stillage and/or syrup/evaporated centrate from a fermentation product production process by adding a thermostable protease to the whole stillage, thin stillage and/or syrup.

The present disclosure provides a novel and gentle process for producing oat-based products containing oat proteins that have good functional properties, including solubility, emulsifying, foaming and gelling properties. This process includes hydrolysis to break down carbohydrates, physical separation to remove insoluble fibers, and membrane filtration to concentrate oat proteins by the removal of sugars. The method can include providing an oat mixture; hydrolyzing the oat mixture with an enzyme or a combination of enzymes; physically separating an insoluble material from the hydrolyzed oat mixture to form a soluble hydrolyzed oat mixture; applying membrane filtration to the soluble hydrolyzed oat mixture using a membrane having molecular weight cut-offs (MWCO) greater than 100 kDa.

Described are polypeptides having glucoamylase activity, compositions comprising such polypeptides, and methods of using such polypeptides and compositions.

The present invention relates to a process for the preparation of a fermentable sugar composition, wherein a polysaccharide composition is treated by at least one glucoamylase enzyme and at least one oligo-1,6-glucosidase enzyme, and wherein the fermentable sugars are removed during the process. Thereby, the otherwise non-fermentable sugars can be utilized in the fermentation process to yield a fermentation product such as an alcohol or an organic acid or amino acid.

Genetically programmed microorganisms, such as bacteria or virus, pharmaceutical compositions thereof, and methods of modulating and treating cancers are disclosed.

The present invention relates to the animal-free production of animal-derived proteins for human consumption by expression of such proteins, e.g. ovalbumin, in fungal cells. The invention relates to fungal cells modified for the production of animal-derived proteins, such as ovalbumin and to methods wherein these cells are used for the production of animal-derived proteins.

Embodiments of the disclosure include particular amyloglucosidase (AMG) compositions formulated as a nutriceutical or medicinal food, for example. The AMG compositions are formulated at a specific dosage and/or are lacking in one or more toxins or have substantially reduced levels of toxin, such as deoxynivalenol (vomit toxin). The AMG compositions are provided to individuals in need thereof, such as an individual with or at risk for congenital sucrase isomaltase syndrome, functional bowel disorders, small bowel bacterial overgrowth, protein-calorie malnutrition (marasmus), radiochemotherapy-induced mucositis and/or short-gut syndrome.

The present invention provides a method of preparing a sorbitol liquid and a liquid polyol using a maltitol raffinate. The method includes the steps of: obtaining a saccharification liquid by adding glucoamylase to the maltitol raffinate for saccharification; obtaining a fermentation liquid by adding a dry yeast to the above saccharification liquid for fermentation; obtaining the sorbitol liquid and the liquid polyol by performing decolorization filtration and membrane separation for the above fermentation liquid. The present invention can fully utilize the maltitol raffinate to increase its added value. Further, the method is simple, practical and low in costs.

The invention is directed to novel variant glucoamylases.