The present invention relates to xylanase variants, polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; compositions comprising the xylanase variants and methods of using the variants.

The disclosure provides for artificial signal peptides generated by systems and methods utilizing deep learning.

The present invention relates to enzyme compositions for high temperature saccharification of cellulosic material and to uses thereof.

The present invention is directed to a yeast strain, or strains, secreting a full suite, or any subset of that full suite, of enzymes to hydrolyze corn starch, corn fiber, lignocellulose, (including enzymes that hydrolyze linkages in cellulose, hemicellulose, and between lignin and carbohydrates) and to utilize pentose sugars (xylose and arabinose). The invention is also directed to the set of proteins that are well expressed in yeast for each category of enzymatic activity. The resulting strain, or strains can be used to hydrolyze starch and cellulose simultaneously. The resulting strain, or strains can be also metabolically engineered to produce less glycerol and uptake acetate. The resulting strain, or strains can also be used to produce ethanol from granular starch without liquefaction. The resulting strain, or strains, can be further used to reduce the amount of external enzyme needed to hydrolyze a biomass feedstock during an Simultaneous Saccharification and Fermentation (SSF) process, or to increase the yield of ethanol during SSF at current saccharolytic enzyme loadings. In addition, multiple enzymes of the present invention can be co-expressed in cells of the invention to provide synergistic digestive action on biomass feedstock. In some aspects, host cells expressing different heterologous saccharolytic enzymes can also be co-cultured together and used to produce ethanol from biomass feedstock.

The present invention relates to processes for producing fermentation products from starch-containing material, wherein a thermostable xylanase that is resistance to inhibition by metal ions in the liquefying starch-containing material is present and/or added during liquefaction.

A method for enhancing the enzymatic efficiency of an enzyme added to poultry feed for a living subject, comprises adding a cellulose-degrading enzyme to a mobile enzyme sequestration platform (MESP) so as to form an enzyme-MESP complex; adding the enzyme-MESP complex to poultry feed for a living subject; the enzyme efficiency of the cellulose-degrading enzyme of the enzyme-MESP complex after being exposed to a first adverse environment for a first period of time is at least 50% higher than the enzyme efficacy of the cellulose-degrading enzyme independent of the MESP being exposed to the first adverse environment for the first period of time.

The present invention relates to xylanase variants of a parent xylanase having increased thermostability when compared to the parent xylanase. The present invention also relates to polynucleotides encoding the variants, nucleic acid constructs, vectors, and host cells comprising the polynucleotides, and method of producing the variants of the present invention.

Provision of a modified promoter derived from a xylanase promoter. A modified promoter comprising a polynucleotide of Xyn3 promoter comprising a polynucleotide that comprises at least one polynucleotide of Xyn1 promoter cis-element or a complementary strand thereof in a region corresponding to the nucleotides at position 374 to 401 of SEQ ID NO:1. The polynucleotide of Xyn3 promoter consist of the following nucleotide sequences: the nucleotide sequence represented by SEQ ID NO:1; the nucleotide sequence represented by the nucleotides at position 350 to 1084 of SEQ ID NO:1; or a nucleotide sequence that has an identity of at least 90% therewith and that comprises the sequence represented by SEQ ID NO:2 in a region corresponding to the nucleotides at position 374 to 401 of SEQ ID NO:1. The polynucleotide of Xyn1 promoter cis-element consists of the nucleotide sequence represented by SEQ ID NO:4.

Provided are methods of making and using an antimicrobial composition, optionally including adding one or more enzymes to a suspension of lees, wherein the lees was formed by fermenting fruit with yeast and the one or more optional enzymes comprise a protease, a carbohydrase, or a combination of a protease and a carbohydrase; and forming a dried lees by drying the lees. In some examples the fruit includes chardonnay grapes, pinot noir grapes, cabernet franc grapes, or a combination of any two or more of chardonnay grapes, pinot noir grapes, and cabernet franc grapes; the yeast includes one or more Saccharomyces cerevisiae strains of yeast; and drying the lees by heating it. In some examples, trub made from fermenting a grain such as barley is used instead of lees. An antimicrobial composition made as provided may be administered to an animal to inhibit bacterial growth.

Methods for decreasing harmful effects of blue light on skin are disclosed that include topically administering to a subject in need thereof a composition of an effective quantity of a purified, enzymatic hydrolysate of Theobroma cacao L. beans comprising peptides and saccharides having a molecular weight between 200 Da and 10 kDa in a physiologically acceptable medium. The hydrolysate of Theobroma cacao L. beans is present in the composition at a concentration from 0.001 to 20% with respect to the total weight of the composition. The composition can be a cosmetic composition.