The present invention relates to a method for chemical production of 3,6-anhydro-L-galactitol (L-AHGoI), which is a novel sugar alcohol, and agarobititol (ABol), which is a disaccharide having the same agarobititol as a reductant end thereof, from sea algae.

The present invention provides Streptomyces coelicolor strain A3(2)_M22-2C43 obtained by inducing a point mutation in the base sequence of the DagB gene in a wild-type Streptomyces coelicolor strain A3(2) by UV radiation. Since the Streptomyces coelicolor strain A3(2)_M22-2C43 according to the present invention expresses a DagB mutant enzyme expressing little or no DagB beta-agarase or exhibiting little or no beta-agarase activity, there is no need for separate isolation and purification of DagA enzymes from culture fluid, and the culture fluid of the Streptomyces coelicolor strain A3(2)_M22-2C43 or supernatant thereof may be used to produce, from agar or agarose, neoagarose oligosaccharides with a higher content of neoagarotetraose or neoagarohexaose than that of neoagarobiose.

The present disclosure relates to an enzyme complex of arabinose isomerase, agarase and 3,6-anhydro galactosidase and a method for producing tagatose by degrading agar using the same. By using the enzyme complex according to the present disclosure, agar obtained from marine biomass can be degraded effectively and useful physiologically active substances such as tagatose can be obtained effectively therefrom.

The present invention relates to a method for producing marine algae-derived agarotriose, and a use thereof as a prebiotic. More specifically, the present invention investigates the characteristics of agarotriose as a prebiotic which is selectively metabolized by probiotic microorganisms, thereby enabling agarotriose to be used as an anti-cancer or anti-inflammatory agent in the fields of food and pharmaceuticals, and enabling agarotriose to be obtained at high yield through efficient purification with minimal loss after enzymatic hydrolysis of a red algae-derived polysaccharide without pre-treatment.

The present invention relates to an enzyme complex which the following (i) to (iv) are combined: (i) chimeric beta-agarase formed by a fusion of beta-agarase and the dockerin module of endo-β-1,4-glucanase-B; (ii) chimeric kappa-carrageenase formed by a fusion of kappa-carrageenase and the dockerin module of endo-β-1,4-glucanase-B; (iii) chimeric anhydro-galactosidase formed by a fusion of anhydro-galactosidase and the dockerin module of endoβ-1,4-glucanase-B; and (iv) mini cellulose-binding protein A, and to a method of degrading red algal biomass using the same. According to the present invention, an enzymatic degradation process is applied for the production of agar degradation products, deviating from conventional methods that relied on physical and chemical pretreatment processes. Thus, the present invention will greatly contribute to efficiently converting marine algae into valuable products by use of a convenient, cost-effective, highly efficient and environmentally friendly degradation system while controlling the products.

The present invention relates to a method for producing marine algae-derived agarotriose, and a use thereof as a prebiotic. More specifically, the present invention investigates the characteristics of agarotriose as a prebiotic which is selectively metabolized by probiotic microorganisms, thereby enabling agarotriose to be used as an anti-cancer or anti-inflammatory agent in the fields of food and pharmaceuticals, and enabling agarotriose to be obtained at high yield through efficient purification with minimal loss after enzymatic hydrolysis of a red algae-derived polysaccharide without pre-treatment.

The present invention relates to a method for chemical production of 3,6-anhydro-L-galactitol (L-AHGoI), which is a novel sugar alcohol, and agarobititol (ABol), which is a disaccharide having the same agarobititol as a reductant end thereof, from sea algae.

The present invention relates to a novel method for purifying 3,6-anhydro-L-galactose by using microorganisms and provides an effect of improving the production yield of 3,6-anhydro-L-galactose by using microorganisms during purification after enzymatic hydrolysis of agarose or agar.

The present invention relates to an expansin-agarase enzyme complex and a method of degrading agar by using the same. The use of the enzyme complex according to the present invention can efficiently degrade agar obtained from marine biomass, and thus can efficiently provide not only galactose or glucose, necessary for ethanol production, but also useful biologically active substances, such as diose, triose, and oligosaccharides.

Provided in the present invention is a novel use of composition containing a neoagarooligosaccharide mixture as an active ingredient. The neoagarooligosaccharide mixture and the like as an active ingredient of the composition according to the present invention can reduce the expression of RANKL, an osteoclast inducer, or IL-6, an inflammatory cytokine, and effectively inhibit differentiation of monocytes into osteoclasts. Thus, the composition according to the present invention may be used as a medicine or functional food for preventing, alleviating, or treating arthritis (especially rheumatoid arthritis). Further, the active ingredient of the composition according to the present invention can be obtained by an enzyme reaction between a readily available substrate such as agar or agarose and DagA as a beta-agarase derived from Streptomyces coelicolor. Thus, it can be produced at a relatively low cost and can easily be ingested by anyone because it has no side effects.