The present invention relates to a genetically engineered microorganism expressing (i) formate tetrahydrofolate (THF) ligase, methenyi-THF cyclohydrolase and methylene-THF dehydrogenase, (ii) the enzymes of the glycine cleavage system (GCS), (iii) serine deaminase and serine hydroxymethyltransferase (SHMT), (iv) an enzyme increasing the availability of NADPH, and (v) optionally formate dehydrogenase (FDH), and wherein the genetically engineered microorganism has been genetically engineered to express at least one of the enzymes of (i) to (v), wheren said enzyme is not expressed by the corresponding microorganism that has been used to prepare the genetically engineered microorganism, and wherein the enzymes of (i) to (v) are genomically expressed.

Disclosed is a recombinant microorganism capable of growing using only carbon dioxide and formic acid by introducing and improving a metabolic pathway for synthesizing pyruvic acid from carbon dioxide and formic acid to enhance pyruvic acid synthesis efficiency and performing additional genetic manipulation, and a method for producing useful substances using the same. Advantageously, the recombinant microorganism is capable of synthesizing pyruvic acid, a C3 organic compound, at a remarkably improved rate, and in particular, grows well even in a medium containing only carbon dioxide and formic acid as carbon sources without a glucose supply, and is thereby capable of synthesizing pyruvic acid and various high value-added compounds using the same as an intermediate product in an economically efficient manner.

The present invention relates to a recombinant microorganism having heterologous genes introduced thereto and a method for producing a useful material from formic acid and carbon dioxide using the microorganism. The present invention provides a novel microorganism having a cyclic metabolic pathway introduced thereto through which C3 or higher carbon organic compounds can be synthesized from formic acid and carbon dioxide, whereby carbon dioxide rich in nature and formic acid that is of low toxicity and suitable for anabolic reaction in view of reaction kinetics and which can be easily and rapidly synthesized from carbon dioxide can be used to effectively synthesize the C3 organic compound pyruvic acid from which various high-value added compound can be synthesized.

Disclosed is a recombinant microorganism capable of growing using only carbon dioxide and formic acid by introducing and improving a metabolic pathway for synthesizing pyruvic acid from carbon dioxide and formic acid to enhance pyruvic acid synthesis efficiency and performing additional genetic manipulation, and a method for producing useful substances using the same. Advantageously, the recombinant microorganism is capable of synthesizing pyruvic acid, a C3 organic compound, at a remarkably improved rate, and in particular, grows well even in a medium containing only carbon dioxide and formic acid as carbon sources without a glucose supply, and is thereby capable of synthesizing pyruvic acid and various high value-added compounds using the same as an intermediate product in an economically efficient manner.

The present invention relates to a recombinant microorganism having heterologous genes introduced thereto and a method for producing a useful material from formic acid and carbon dioxide using the microorganism. The present invention provides a novel microorganism having a cyclic metabolic pathway introduced thereto through which C3 or higher carbon organic compounds can be synthesized from formic acid and carbon dioxide, whereby carbon dioxide rich in nature and formic acid that is of low toxicity and suitable for anabolic reaction in view of reaction kinetics and which can be easily and rapidly synthesized from carbon dioxide can be used to effectively synthesize the C3 organic compound pyruvic acid from which various high-value added compound can be synthesized.

An isolated microorganism that expresses enzymes of the reductive glycine pathway is disclosed. The microorganism is capable of converting formate to pyruvate or glycerate via the formation of glycine and serine. Methods of generating same are further described.

An exemplary embodiment of the present disclosure provides a method of converting formate to a desired compound. The method comprises providing a biocatalyst and formate to form a reaction mixture and reacting at least the biocatalyst with formate to produce a first reaction product.