A method for preparation of and use of R-hydroxynitrile lyase derived from mango. Specifically, a variety of mutant cyanohydrin lyases were obtained by constructing random mutation and site-saturation mutagenesis libraries and high-throughput screening, and the method was safe, simple and easy to operate.

Methods for synthesizing and using R-hydroxynitrile lyase derived from mango. Specifically, a variety of mutant cyanohydrin lyases were obtained by mutating a wild-type hydroxynitrile lyase gene, adding an enzyme cleavage site thereto, preparing a recombinant plasmid by inserting the mutated wild-type hydroxynitrile lyase gene into a vector, introducing the recombinant plasmid into a strain of bacteria, and secreting and expressing the R-hydroxynitrile lyase in the strain. The method Is safe, simple and easy to operate.